Aged Garlic Extract Scavenges Superoxide Radicals |
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Authors: | Naoaki Morihara Minoru Hayama Hirotada Fujii |
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Institution: | (1) Healthcare Research Institute, Wakunaga Pharmaceutical Co. Ltd., 1624 Shimokotachi, Kodacho, Akitakata, Hiroshima 739-1195, Japan;(2) School of Health Sciences, Sapporo Medical University, Sapporo, Japan |
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Abstract: | There is increasing evidence to suggest that many degenerative or pathological processes, such as aging, cancer, and coronary
heart disease, are related to reactive oxygen species and radical-mediated reactions. We examined the effectiveness of aged
garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, and its component for scavenging of superoxide
by using the hypoxanthine-xanthine oxidase and human neutrophils. In the hypoxanthine-xanthine oxidase system, electron spin
resonance showed that aged garlic extract scavenged superoxide radicals in a dose-dependent manner up to 54%. The EC50 value of aged garlic extract for the superoxide radical scavenging effect was 0.80 mg/ml. N-α-(1-deoxy-D-fructos-1-yl)-L-arginine
(25.9%) and (1S, 3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-1,3-dicarboxylic acid (20.8%), water-soluble moieties of AGE,
also exerted superoxide scavenging effects. Phorbol 12-myristate 13-acetate-activated human neutrophils produced superoxide
radical of 56.6 ± 9.27 nmol/min/107 cells. Aged garlic extract (3 mg/ml) significantly inhibited superoxide production in comparison to the control. These data
suggest that aged garlic extract may be useful for preventing diseases associated with reactive oxygen species. |
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