The Antioxidant and Anti-proliferative Activity of the Lebanese <Emphasis Type="Italic">Olea europaea</Emphasis> Extract |
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Authors: | Rida?Fares Samer?Bazzi Serine?E?Baydoun Email author" target="_blank">Roula?M?Abdel-MassihEmail author |
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Institution: | (1) Department of Biology, University of Balamand, Al-Koura, Tripoli, 100, Lebanon;(2) Department of Biology, American University of Beirut, Beirut, Lebanon; |
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Abstract: | It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study,
the anti-proliferative activity of plant extracts from olive (Olea europaea L.) leaves was tested on human leukemic cell line (Jurkat). Cytotoxicity of various concentrations of plant extracts was examined
and the IC50 was determined. Olive leaf extracts showed concentration-dependent anti-proliferative effect as determined by the WST-1 proliferation
kit and 3H]-thymidine incorporation method. To study whether cell death was due to apoptosis, cells were stained with Annexin V-FITC
and PI and the expression of important regulatory proteins (Bcl-2, Bax, and p53) involved in apoptosis were examined by Western
blot. The antioxidant activity of olive leaves (SC50 = 0.1 mg dry weight) was studied using the DPPH scavenging method. Present findings suggest that olive leaves extracts exhibit
anti-proliferative effect on leukemic cells by inducing apoptosis. |
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