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| 大鼠心肌细胞条件培养基对兔胚胎干细胞分离培养效果的影响 | |
| 引用本文： | 郭志林,王新庄,张 涌.大鼠心肌细胞条件培养基对兔胚胎干细胞分离培养效果的影响[J].西北农林科技大学学报(社会科学版),2007,35(4):1-5. |
| 作者姓名： | 郭志林 王新庄 张 涌 |
| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西,杨凌,712100;河南省肉牛工程技术研究中心,河南,郑州,450003 2. 河南省肉牛工程技术研究中心,河南,郑州,450003 3. 西北农林科技大学,动物科技学院,陕西,杨凌,712100 4. 河南省肉牛工程技术研究中心,河南,郑州,450003;河南农业大学,牧医工程学院,河南,郑州,450002 |
| 基金项目： | 河南省高校杰出科研创新人才工程项目 |
| 摘 要： | 采用大鼠心肌细胞条件培养基对兔胚胎干细胞(Embryonic stem cells,ESC)进行分离、传代培养,研究大鼠心肌细胞条件培养基对兔ESC分离培养效果的影响。结果显示,用SD大鼠心肌细胞条件培养基培养的兔ESC集落呈岛屿状生长,碱性磷酸酶染色呈强阳性,体外分化可形成类胚体状结构,贴壁的类胚体周边会出现许多分化的上皮样细胞或单个散在的细胞;常规冻存后再传代的兔ESC集落具有较为一致的生长特征,并呈现胚胎干细胞集落所特有的岛屿状生长形态;传至第5代的兔ESC集落核型正常率>75％。表明SD大鼠心肌细胞条件培养基可用于兔胚胎干细胞分离培养。 |
| 关 键 词： | 兔 细胞培养 大鼠心肌细胞条件培养基 类胚胎干细胞 |
| 文章编号： | 1671-9387(2007)04-0001-05 |
| 收稿时间： | 2006/10/30 0:00:00 |
| 修稿时间： | 2006-10-30 |
Effect on isolation and culture of rabbit embryonic stem cells with rat cardiomyocyte conditioned medium |
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| GUO Zhi-lin,WANG Xin-Zhuang,ZHANG Yong,WANG Mu,WANG Yi-Min,HU Wen-Ju.Effect on isolation and culture of rabbit embryonic stem cells with rat cardiomyocyte conditioned medium[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(4):1-5. | |
| Authors: | GUO Zhi-lin WANG Xin-Zhuang ZHANG Yong WANG Mu WANG Yi-Min HU Wen-Ju |
| Institution: | (College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi 712100,China; The Research Center of Cattle Engineering Technology in He’nan,Zhengzhou,He’nan 450003,China; The College of Animal Science and Veterinary,He’nan Agricultural University,Zhengzhou ,He’nan 450002,China) |
| Abstract: | Effect on isolation and culture of rabbit embryonic stem (ES) cells with rat cardiomyocyte conditioned medium was studied. Results showed that isolated rabbit ES-like cells clusters cultured by SD rat cardiomyocyte conditioned medium were island-like;alkaline phosphatase staining of rabbit ES-like cell was strongly positive; rabbit ES-like cells could become embryonic bodies-like structures in vitro,and embryonic bodies which stuck to plates could be differentiated into epithelial-like cells or scattered single cell;rabbit ES cells cluster were identical island-like image and grew coincidently by culture after routin freeze; karyotype analysis of the 5th passage rabbit ES cells was researched, and the karyotype normal rate was over 75%. The essay showed SD rat cardiomyocyte conditioned medium can be used to isolation and culture of rabbit ES-like cell. |
| Keywords: | rabbit cells culture rat cardiomyocytes conditioned medium embryonic stem-like cells |
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