几种因素对东革阿里的愈伤组织中抗肿瘤药物成分累积的影响(英文)

研究提高东葛阿里的愈伤组织中抗肿瘤药物成分的累积。有些因素(不同培养基成分和物理因素)影响东葛阿里的愈伤组织中抗肿瘤药物成分的积累。结果表明:在1/4MS基本培养基的愈伤组织中获得较高的抗肿瘤药物成分(3.84mg·g-1干重)。与在基本培养基中添加碳源相比,添加2%果糖,抗肿瘤药物成分产量最高(4.59mg·g-1)。添加激素(3,6-Dichloro-o-aniscicacid,2.0mg·L-1),也可提高抗肿瘤药物成分产量(12.3mg·g-1)。在基本培养基pH值为5.5时,可获得较高的抗肿瘤药物成分产量(1.53mg·g-1)。当基本培养基附加苯丙氨酸(1×10-1μM)时,抗肿瘤药物成分产量也被提高到2.34mg·g-1。成功地提取了在试管培养下获得的抗肿瘤药物成分,具有大范围生产的潜力。

Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia

A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg(g-1 DW (Dry Weight)) is obtained from callus cultured in 1/4 MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg(g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg(L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg(g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg(g-1 DW). Production of 9-methoxycanthin-6-one (2.34 mg(g-1 DW) in callus cultures is also increased when the medium is added with 1(10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology.