| 鸭疫里氏杆菌生长曲线的测定 |
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| 引用本文： | 胡清海,陈鸿军,刘晓文,章明,张知良,丁铲,章耀良. 鸭疫里氏杆菌生长曲线的测定[J]. 畜牧与兽医, 2002, 34(8): 8-9 |
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| 作者姓名： | 胡清海 陈鸿军 刘晓文 章明 张知良 丁铲 章耀良 |
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| 作者单位： | 1. 江苏省家禽科学研究所,江苏,扬州,225003
2. 江苏省江阴市青阳镇畜牧兽医站,江苏,江阴,214401 |
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| 基金项目： | 江苏省“九五”农业科技攻关项目 (BE9932 4 ) |
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| 摘 要： | 采用P L琼脂平板涂布法 ,测定鸭疫里氏杆菌WJ4株 (血清 1型 )、FXb7株 (血清 2型 )在P L肉汤中静置培养和振荡培养情况下的生长曲线。结果表明 ,在P L肉汤中振荡培养时 ,WJ4株培养 10h达生长最高峰 ( 4 7× 10 1 0 CFU mL) ,FXb7株 11h活菌数最高达 ( 4 2× 10 1 0 CFU mL) ;在静置培养时 ,WJ4株培养 2 2h达最高峰 ( 1 8× 10 9CFU mL) ,FXb7株 2 3h可达最高峰 ( 1 3× 10 9CFU mL)。
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| 关 键 词： | 鸭疫里氏杆菌 生长曲线 平板稀释涂布法 |
| 文章编号： | 0529-5130(2002)08-0008-02 |
| 修稿时间： | 2002-01-04 |
Determination of growth curve of Riemerella anatipestifer |
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| HU Qing hai,CHEN Hong jun,LIU Xiao wen,et al. Determination of growth curve of Riemerella anatipestifer[J]. Animal Husbandry & Veterinary Medicine, 2002, 34(8): 8-9 |
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| Authors: | HU Qing hai CHEN Hong jun LIU Xiao wen et al |
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| Abstract: | Riemerella anatipestifer serotype 1 strain WJ 4 and serotype 2 strain FXb 7 were inoculated into sterile P L broth medium.Their growth curves were determinated under shaking and stationary culture respectively.The results showed that WJ 4 strain grew to peak at 10 h when inocubated in P L broth at 37℃ on the shaker(4 7×10 10 CFU/mL),and reached the peak at 23 h(1 8×10 9 CFU/mL)under stationary culture.For FXb 7 strain,the number or cells reached the peak at 11 h(4 2×10 10 CFU/mL)and 23 h(1 3×10 9 CFU/mL),respectively. |
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| Keywords: | Riemerella anatipestifer growth curve dilution plating procedure |
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