Interspecific hybrids betweenAllium fistulosumandAllium schoenoprasumreveal carotene-rich phenotype |
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Authors: | Mikihisa Umehara Takayuki Sueyoshi Katsumi Shimomura Masatoshi Iwai Masayoshi Shigyo Keita Hirashima Takao Nakahara |
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Affiliation: | (1) Department of Biotechnology, Fukuoka Agricultural Research Center, Yoshiki 587, Chikushino Fukuoka, 818-8549, Japan;(2) Department of Vegetable Breeding, Fukuoka Agricultural Research Center, Yoshiki 587, Chikushino Fukuoka, 818-8549, Japan;(3) Faculty of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan |
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Abstract: | Interspecific hybridization is an effective method to generate a new crop that gains available functions in a short time. Interspecific hybrids (2n= 16) betweenAllium fistulosumL. (2n= 16) andAllium schoenoprasumL. (2n= 16) were produced by reciprocal crossings through ovary culture, but the hybrids were much fewer in the combination usingA. schoenoprasumas a seed plant. All the hybrids have eight long chromosomes originated fromA.fistulosumand eight short chromosomes originated fromA. schoenoprasum. In addition, the hybridity was confirmed by randomly amplified polymorphic DNA (RAPD) analysis and cleaved amplified polymorphic sequence (CAPS) analysis of the rDNA internal transcribed spacer (ITS) region. The interspecific hybrids showed a vigorous growth habit; their foliage was slightly bloomy and deep green. The hybrids did not form bulbs, but rather propagated vegetatively by tillering. Carotene contents of the hybrids and both parents were quantified using high-performance liquid chromatography (HPLC). The contents of all edible parts of the hybrids were approximately seven times higher than those of either parent. These results indicate that the hybrid is a new and carotene-rich vegetable ofAlliumspecies. |
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Keywords: | Allium fistulosumL. Allium schoenoprasumL. interspecific hybrid ovary culture carotenoid |
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