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| 棉花GbGAI2启动子克隆及表达分析 | |
| 引用本文： | 于晓玲,崔百明,温玮,王树昌,彭明. 棉花GbGAI2启动子克隆及表达分析[J]. 热带作物学报, 2010, 31(8): 1272-1279. DOI: 10.3969/j.issn.1000-2561.2010.08.007 |
| 作者姓名： | 于晓玲 崔百明 温玮 王树昌 彭明 |
| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海南海口,571101;海南大学农学院,海南儋州,571737 2. 石河子大学,新疆石河子,832003 3. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
| 基金项目： | 国家重点基础研究发展计划(973)项目，转基因生物新品种培育重大专项 |
| 摘 要： | 通过染色体步移方法获得棉花GbGAI2基因的上游2378bp启动子序列,并对其进行生物信息学分析。将启动子序列与GUS基因连接,转化拟南芥,并对转化植株进行组织化学分析。结果表明,启动子区域内含有丰富的与光信号途径、激素信号途径(GA/IAA)、逆境胁迫相关等的顺式作用元件。在外施GA条件下,启动子活性增强。通过对启动子表达模式的分析表明,棉花GbGAI2基因在GA信号途径中起很大作用。 |
| 关 键 词： | DELLA蛋白 启动子 基因克隆 表达分析 |
Cloning and Function Analysis of the Gene Promoter of GbGAI2 in Cotton |
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| Yu Xiaoling,Cui Baiming,Wen Wei,Wang Shuchang and Peng Ming. Cloning and Function Analysis of the Gene Promoter of GbGAI2 in Cotton[J]. Chinese Journal of Tropical Crops, 2010, 31(8): 1272-1279. DOI: 10.3969/j.issn.1000-2561.2010.08.007 | |
| Authors: | Yu Xiaoling Cui Baiming Wen Wei Wang Shuchang Peng Ming |
| Affiliation: | 1 Institute of Tropical Bioscience and Biotechnology, CATAS 2 College of Agriculture, Hainan University;Shihhotze University, Shihhotze;1 Institute of Tropical Bioscience and Biotechnology, CATAS 2 College of Agriculture, Hainan University;Institute of Tropical Bioscience and Biotechnology, CATA;Institute of Tropical Bioscience and Biotechnology, CATA |
| Abstract: | The 2 378 bp promoter sequence in the upstream of the GbGAI2 gene from cotton was cloned by genome walking and the bioinformatics of the promoter was analyzed. The promoter: GUS expression vector was constructed and transformed into Arabidopsis. The transgenic Arabidopsis plants were analyzed through histochemistry. The results showed that the promoter contained a variety of the cis-elements related to light signaling pathways, hormone signaling pathways(GA/IAA), and other stress-responsive elements. The activity of the promoter increased under GA exogenous. The expression patterns of the promoter showed that cotton GbGAI2 gene played an important role in GA signaling pathway. |
| Keywords: | DELLA protein promoter Gene cloning Expression analysis |
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