| 一株解脂耶氏酵母菌的鉴定及其系统发育树分析 |
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| 引用本文： | 徐同宝,李吕木,蔡海莹,甄长风,孙林,付弘贇. 一株解脂耶氏酵母菌的鉴定及其系统发育树分析[J]. 安徽农学通报, 2008, 14(13): 29-30 |
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| 作者姓名： | 徐同宝 李吕木 蔡海莹 甄长风 孙林 付弘贇 |
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| 作者单位： | 安徽农业大学动物科技学院,安徽合肥,230036;安徽长风农牧科技有限公司,安徽长丰,231100 |
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| 基金项目： | 安徽省科技攻关项目，合肥市重点农业科技项目 |
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| 摘 要： | 从未发酵猪粪中分离出一株酵母样菌株7B3,用BioMerieux Vitek公司的酵母样生化鉴定卡（yeast biochemical card）鉴定该菌为解脂耶罗维亚酵母。为了进一步确定该菌的分类学地位,测定了其18S rDNA序列,分析了相关菌株的同源性,构建其系统发育树。结果表明,该菌株与Yarrowia Lipolytica M相似度最高。
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| 关 键 词： | 解脂耶罗威亚酵母 鉴定 酵母生化鉴定卡 18S’rDNA |
| 文章编号： | 1007-7731(2008)13-29-02 |
| 修稿时间： | 2008-04-30 |
Identification and phylogenetic tree Analysis of Yarrowia Lipolytica |
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| Xu Tongbao,LI Lumu,Cai Haiying,Zhen Changfeng,Sun Lin,Fu Hongyun. Identification and phylogenetic tree Analysis of Yarrowia Lipolytica[J]. Auhui Agricultural Science Bulletin, 2008, 14(13): 29-30 |
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| Authors: | Xu Tongbao LI Lumu Cai Haiying Zhen Changfeng Sun Lin Fu Hongyun |
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| Affiliation: | Xu Tongbao, Li Lumu, Cai Haiying,Zhen Changfeng, Sun Lin, Fu Hongyun ( 1. School of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 China; 2 Anhui Changfeng Agricultrue -Livestock Sicence and Technology Co. Ltd. , Hefei 231100, China) |
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| Abstract: | A yeast-like strain 7B3 was isolated from pig manure, it grows well in manure.It was identified by Yarrowia lipolytica with the yeast biochemical card(YBC) of bioMerieux Vitek Company.To determine the systematics rank of this strain furthermore,18S rRNA encoding gene(18S rDNA)from 7B3 was amplified,and its nucleotide sequence was determined.Homology of similar strains was analyzed,and phylogenetic tree was constructed.The results show that the strain was similar to Yarrowia Lipolytica M. |
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| Keywords: | 18S'rDNA |
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