托里桉组培快繁技术初步研究 |
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引用本文: | 林文革,陈国彪,洪嫦莉,张其生,洪长福. 托里桉组培快繁技术初步研究[J]. 桉树科技, 2015, 0(4): 32-35. DOI: 10.3969/j.issn.1674-3172.2015.04.007 |
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作者姓名: | 林文革 陈国彪 洪嫦莉 张其生 洪长福 |
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作者单位: | 1. 漳州市地缘生物技术有限公司,福建 漳州,363000;2. 福建省龙海九龙岭国有林场,福建 龙海,363112;3. 长泰县园林管理处,福建 长泰,363900;4. 漳州市速生丰产林基地管理中心,福建 漳州,363000 |
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摘 要: | 以2 a托里桉超级苗不同株系的茎段为外植体进行组培快繁研究,结果表明:适合诱导的培养基为MS改良+6-BA 0.5mg·L-1+NAA 0.1 mg·L-1;适合6号株系增殖培养基为MS改良+6-BA 0.2 mg·L-1+NAA 0.1 mg·L-1,适合7号、10号株系的增殖培养基为MS改良+6-BA 0.3 mg·L-1+NAA 0.2 mg·L-1;适当缩短继代周期是解决桉苗顶稍枯萎、上部叶片脱落等症状简单、有效的方法;适合的生根培养基为1/2MS改良+ IBA 0.2 mg·L-1+ ABT 0.6 mg·L-1,生根苗移栽成活率可达90%。
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关 键 词: | 托里桉 组培 快速繁殖 株系 |
Primary Study on Rapid Propagation of Corymbia torelliana |
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Abstract: | Stem segments from two-year-old superior seedlings of different varieties of Corymbia torelliana were used as explants to study rapid propagation of this species through tissue culture. The results showed that best medium of inducement was modified MS with 0.5 mg·L-1 6-BA and 0.1 mg·L-1 NAA. The best medium for subculture proved to be modified MS with 0.2 mg·L-1 6-BA and 0.1 mg·L-1 NAA for variety No.6 and modified MS with 0.3 mg·L-1 6-BA and 0.2 mg·L-1 NAA for varieties No.7 and No.10. A simple and effective method to solve the phenomenon of withering of the top slightly and dropping of the upper leaves involved shortening the subculture cycle. The best medium for rooting proved to be half modified MS with 0.2 mg·L-1 IBA and 0.6 mg·L-1 ABT. Using the media for tissue culture, the transplant survival rates of rooted seedlings could reach 90%. |
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Keywords: | Corymbia torelliana tissue culture rapid propagation lines |
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