| 甜菜转基因体系的相关影响因素研究 |
| |
| 引用本文： | 鲁振强,陈艳平,马龙彪,佟清越,陈连江.甜菜转基因体系的相关影响因素研究[J].中国糖料,2010(3):1-3. |
| |
| 作者姓名： | 鲁振强 陈艳平 马龙彪 佟清越 陈连江 |
| |
| 作者单位： | 1. 黑龙江省普通高等学校生化与分子生物学重点实验室/黑龙江大学生命科学学院,哈尔滨,150080
2. 黑龙江省普通高等学校甜菜遗传育种重点实验室/黑龙江大学农作物研究院,哈尔滨,150080 |
| |
| 基金项目： | 中国博士后科学基金面上资助项目，黑龙江省博士后基金资助项目，黑龙江省教育厅资助项目，黑龙江大学青年基金项目 |
| |
| 摘 要： | 以16种基因型的二倍体甜菜(Beta vulgaris L.)为材料,建立并优化了甜菜的植株再生体系;对影响甜菜转基因的相关因素进行了比较分析,确定了抗生素标记基因的适宜筛选浓度为200mg/L;菌液适宜浸染浓度OD600为0.3;浸染最适时间为10min;外植体与农杆菌适宜共培养时间为4d;添加乙酰丁香酮的适宜浓度为100μmol/L。研究结果的获得,为完善甜菜的遗传工程以及甜菜基因组学的相关研究奠定基础。
|
| 关 键 词： | 甜菜 转基因 遗传改良 |
Related Impact Factors on Transgene System of Sugarbeet |
| |
| LU Zhen-qiang,CHEN Yan-ping,MA Long-biao,TONG Qing-yue,CHEN Lian-jiang.Related Impact Factors on Transgene System of Sugarbeet[J].Sugar Crops of China,2010(3):1-3. |
| |
| Authors: | LU Zhen-qiang CHEN Yan-ping MA Long-biao TONG Qing-yue CHEN Lian-jiang |
| |
| Institution: | LU Zhen-qiang1,CHEN Yan-ping1,MA Long-biao2,TONG Qing-yue1,CHEN Lian-jiang2(1.Key Lab of Biochemistry and Molecular Biology/College of Life Sciences Heilongjiang University,Harbin 150080,China,2.Key Lab of Crop Genetics and Breeding/Institute for Crop Research,Heilongjiang University,China) |
| |
| Abstract: | Sugarbeet(Beta vulgaris L.) varieties including 16 kinds genotype were chosen,its plant generation system was established and optimized,and related factors on genetic transformation were compared and analyzed as well.In this study,optimal dose of Kanamycin 200 mg/L was identified;optimal infected Agrobacterium tumefaciens EHA105-OsAPX3 was OD600 = 0.3;and the optimal infected internal was 10min;the optimal explants co-cultured with agrobacterium was 4 days;the optimal concentration of acetosyringone added w... |
| |
| Keywords: | Sugarbeet(Beta vulgaris L ) Gene transformation Genetic improvement |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
