| 离体培养条件对百合鳞片辐射敏感性的影响 |
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| 引用本文： | 张冬雪,王丹,张志伟.离体培养条件对百合鳞片辐射敏感性的影响[J].核农学报,2007,21(3):224-228. |
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| 作者姓名： | 张冬雪 王丹 张志伟 |
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| 作者单位： | 西南科技大学生命科学与工程学院,四川,绵阳,621000 |
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| 基金项目： | 中国工程物理研究院基金 |
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| 摘 要： | 研究了辐射处理主因素及其与培养基成分、外植体类型的交互作用对东方百合鳞片辐射敏感性的影响。结果表明:在试验辐射剂量范围内,离体培养约6d的百合鳞片经60Coγ射线辐照后存活率随剂量增大而呈现下降趋势;辐射处理对百合鳞片的出芽率和出芽数有强烈的抑制作用。在芽诱导阶段,培养基成分在一定程度上影响鳞片的辐射敏感性;鳞片辐射敏感性随培养时间增加而减弱;外部鳞片与中部鳞片表现的辐射敏感性基本一致。
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| 关 键 词： | 百合 离体培养 辐射敏感性 γ射线辐照 |
| 文章编号： | 1000-8551（2007）03-224-05 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2006-06-06 |
EFFECTS OF CONDITION IN VITRO ON THE IRRADIATION SENSITIVITY OF SCALES |
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| ZHANG Dong-xue,WANG Dan,ZHANG Zhi-wei.EFFECTS OF CONDITION IN VITRO ON THE IRRADIATION SENSITIVITY OF SCALES[J].Acta Agriculturae Nucleatae Sinica,2007,21(3):224-228. |
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| Authors: | ZHANG Dong-xue WANG Dan ZHANG Zhi-wei |
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| Institution: | Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan 621002 |
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| Abstract: | The effects of irradiation and the interactions between irradiation and the ingredients of culture medium and the type of explants on radiation sensitivity of scales of lily were studied.The results showed that when lily scales were exposed to after cultured in vitro for about six days.The survival rate of scales in vitro decreased with the increase of irradiation dose. Irradiation significantly inhibited the sprouting rate and the number of sprouts of scales in vitro.During the bud induction,the effects of ingredients of culture medium on radiation sensitivity of scales were obvious at certain degree,and also the culture time.Both exterior scales and middle scales appeared an identical irradiation sensitivity. |
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| Keywords: | lily tissue culture irradiation sensitivity γ2rays irradiation |
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