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小麦抗秆锈突变系龙辐03D51的筛选及其抗病性的遗传分析与RAPD标记
引用本文:孙岩,尹静,王广金,张宏纪,黄景华,郭强,刁艳玲,刘东君. 小麦抗秆锈突变系龙辐03D51的筛选及其抗病性的遗传分析与RAPD标记[J]. 核农学报, 2007, 21(2): 120-123
作者姓名:孙岩  尹静  王广金  张宏纪  黄景华  郭强  刁艳玲  刘东君
作者单位:黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086
基金项目:黑龙江省农业科学院基金
摘    要:以龙6239幼胚为外植体,利用辐射诱变和组织培养相结合的方法,选育出突变系龙辐03D51,经秆锈接种鉴定,发现其对优势小种21C3CPH免疫,而亲本龙6239对21C3CPH高度感染。遗传分析表明,抗病性由显性单基因控制。该突变系还具有其亲本的优质、高产特点,已成为优异的后备品系。在RAPD检测中,所用的60个随机引物中有3个引物在龙辐03D51和其亲本龙6239中具有多态性,引物E07、E11、E17在龙辐03D51中分别扩增出380bp、700bp和600bp的特异带,初步认为这些谱带可能与秆锈抗性有关。

关 键 词:小麦  秆锈抗性  体细胞无性系变异  遗传分析  RAPD
文章编号:1000-8551(2007)02-120-04
收稿时间:2009-12-31
修稿时间:2006-06-09

BREEDING WHEAT MUTANT LONGFU 03D51 WITH RESISTING STEM RUST AND GENETIC ANALYZATION OF RESISTING DISEASE AND RAPD MAKER
SUN Yan,YIN Jing,WANG Guang-jin,ZHANG Hong-ji,HUANG Jing-hua,GUO Qiang,DIAO Yan-ling,LIU Dong-jun. BREEDING WHEAT MUTANT LONGFU 03D51 WITH RESISTING STEM RUST AND GENETIC ANALYZATION OF RESISTING DISEASE AND RAPD MAKER[J]. Acta Agriculturae Nucleatae Sinica, 2007, 21(2): 120-123
Authors:SUN Yan  YIN Jing  WANG Guang-jin  ZHANG Hong-ji  HUANG Jing-hua  GUO Qiang  DIAO Yan-ling  LIU Dong-jun
Affiliation:Crop Breeding Institute of Heilongfiang Academy of Agricultural Sciences , Harbin, Heilongfiang 150086
Abstract:Longfu 03D51 was bred using Long 6239 immature embryo as explants through radiation mutagenesis breeding coupled with tissue culture technique.By artificial inoculation and field breeding identified Longfu 03D51 was identified with high resistance to dominant strain 21C_3CPH,good quality and high yield.Genetic analysis suggested that stem rust was controlled by a single gene.The DNA polymorphism on Longfu 03D51 and its parents were studied by random amplified polymorphic DNA(RAPD),3 primers out of 60 RAPD primers were found polymorphism between the Longfu 03D51 and its parent Long 6239,and 3 stable polyphymorphic bands amplified in Longfu 03D51 with the size of 380bp,700bp,600bp by random primer E07,E11,E17,respectively.Genetic and RAPD analysis indicated that 3 RAPD primers E07,E11,E17 might be linked to the gene of stem rust resistance.
Keywords:wheat  resistibility to stem rust  somaclonal variation  genetic analysis  RAPD
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