| 甘蓝型油菜小孢子离体诱变——紫外线对小孢子胚状体再生的影响 |
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| 引用本文： | 石淑稳,吴江生,牛勤思.甘蓝型油菜小孢子离体诱变——紫外线对小孢子胚状体再生的影响[J].核农学报,2007,21(1):17-19,43. |
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| 作者姓名： | 石淑稳 吴江生 牛勤思 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,湖北,武汉,430070 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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引进国际先进农业科技计划(948计划)
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创新团队项目 |
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| 摘 要： | 用紫外线对3个甘蓝型油菜品系的离体小孢子进行照射0-60s。未经照射的对照和已照射的小孢子于NLN培养基进行培养,诱导胚状体。结果表明,照射20s和60s对胚状体的再生没有影响,照射40s则有促进作用。
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| 关 键 词： | 甘蓝型油菜 紫外线 离体诱变 小孢子培养 |
| 文章编号： | 1000-8551（2007）01-017-03 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2006-07-20 |
IN VITRO MICROSPORES MUTAGENESIS IN Brassica napus.——RESPONSE OF MICROSPORE EMBRYOGENESIS TO UV RADIATION |
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| SHI Shu-wen,WU Jiang-sheng,NIU Qin-si.IN VITRO MICROSPORES MUTAGENESIS IN Brassica napus.——RESPONSE OF MICROSPORE EMBRYOGENESIS TO UV RADIATION[J].Acta Agriculturae Nucleatae Sinica,2007,21(1):17-19,43. |
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| Authors: | SHI Shu-wen WU Jiang-sheng NIU Qin-si |
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| Institution: | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070 |
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| Abstract: | The isolated microspores from 3 lines of Brassica napus were irradiated with ultraviolet(UV) light for 0～60 seconds and cultured in NLN medium to induce embryogenesis.The results showed that the treatment of 40s produced higher embryo yield than those of 0,20 and 60s,but no significant difference was found among 4 treatments for embryo yield. |
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| Keywords: | Brassica napus ultraviolet light radiation Microspore culture in vitro mutation |
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