| 中慢生型天山根瘤菌CCBAU060A自体诱导物合酶基因的筛选及其在大肠杆菌中的表达 |
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| 引用本文： | 曹慧娟,郑会明,杨梦华,钟增涛,朱军. 中慢生型天山根瘤菌CCBAU060A自体诱导物合酶基因的筛选及其在大肠杆菌中的表达[J]. 土壤学报, 2009, 46(1): 117-121 |
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| 作者姓名： | 曹慧娟 郑会明 杨梦华 钟增涛 朱军 |
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| 作者单位： | 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095 |
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| 基金项目： | 国家自然科学基金，国家杰出青年科学基金 |
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| 摘 要： | 细菌在高细胞密度下可以产生化学信号分子调控细菌相关基因的表达,这种信号分子被称为自体诱导物(Autoinducer,AI)。采用检测菌株JZA1在中慢生型天山根瘤菌CCBAU060A(Mesorhizobium tianshanenseCCBAU060A)培养上清液中检测到了高活性的自体诱导物;利用转座子随机突变的方法获得了AI缺失突变株,并克隆得到了相关的自体诱导物合酶基因;将自体诱导物合酶基因在大肠杆菌中进行表达,对大肠杆菌重组菌株进行自体诱导物检测发现,该合酶基因在大肠杆菌中能够合成四种自体诱导物分子。
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| 关 键 词： | 群体感应 中慢生型天山根瘤菌CCBAU060A 自体诱导物合酶 大肠杆菌重组表达 |
Screening for autoinducer synthase gene in Mesorhizobium tianshanense CCBAU060A and its recombinant expression in Escherichia coli |
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| Cao Huijuan,Zheng Huiming,Yang Menghu,Zhong Zengtao and Zhu Jun. Screening for autoinducer synthase gene in Mesorhizobium tianshanense CCBAU060A and its recombinant expression in Escherichia coli[J]. Acta Pedologica Sinica, 2009, 46(1): 117-121 |
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| Authors: | Cao Huijuan Zheng Huiming Yang Menghu Zhong Zengtao Zhu Jun |
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| Affiliation: | Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University;Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University;Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University;Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University;Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University |
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| Abstract: | |
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| Keywords: | Quorum sensing Mesorhizobium tianshanense AHL synthase Expression |
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