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| 基于稻瘟菌毒性相关基因的指纹类型与致病型关系分析 | |
| 引用本文： | 石军,王志,李仕贵,马炳田.基于稻瘟菌毒性相关基因的指纹类型与致病型关系分析[J].分子植物育种,2008,6(6). |
| 作者姓名： | 石军 王志 李仕贵 马炳田 |
| 作者单位： | 1. 四川农业大学水稻研究所,温江,611130;绵阳市农业科学研究所,绵阳,621023 2. 绵阳市农业科学研究所,绵阳,621023 3. 四川农业大学水稻研究所,温江,611130 |
| 摘 要： | 本研究根据8对稻瘟菌毒性相关基因序列同源性设计其特异性引物，通过PCR扩增分析单孢分离培养的不同地理来源的44个菌株，获得其指纹。利用18个丽江新团黑谷（LTH）单基因鉴别寄主对其中的31个菌株进行致病型鉴定；用SPSS11．5聚类分析，根据31个菌株在18个LTH NILs上的抗感反应，在Lable No．10的位置，将菌株分为7种致病类型。将上述的两种不同的聚类结果进行相关性分析，发现通过毒性相关基因的特异性引物划分出的指纹类型与用菌株在LTH NILs的抗感反应划分出的致病型之间不存在——对应关系。 |
| 关 键 词： | 稻瘟菌 DNA指纹图谱 致病型 鉴别品种 |
Correlation between Pathotypes and DNA Fingerprinting Patterns Based on Virulence Gene of Rice Blast Fungus |
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| Shi Jun,Wang Zhi,Li Shigui,Ma Bingtian.Correlation between Pathotypes and DNA Fingerprinting Patterns Based on Virulence Gene of Rice Blast Fungus[J].Molecular Plant Breeding,2008,6(6). | |
| Authors: | Shi Jun Wang Zhi Li Shigui Ma Bingtian |
| Institution: | Shi Jun 1,2 Wang Zhi 2 Li Shigui 1 Ma Bingtian 1* 1 Rice Research Institute,Sichuan Agricultural University,Wenjiang,611130,2 Mianyang Agricultural Science Research Institute,Mianyang,621023 |
| Abstract: | Based on the homology of the genes relative to virulence of rice blast fungus, eight pairs of specific primers were designed. The fingerprints of 44 were isolated by culturing single spore of rice blast fungus from dif- ferent regions were obtained by PCR. On the other hand, 31 pathotypes of the 44 isolates were identified by inocu- lating 18 monogenic hosts of LTH (Lijiangxintuanheigu) near isogenic lines (NILs). According to the response of re- sistance-susceptibility to 18 monogenic hosts, the 31 isolate... |
| Keywords: | Pyricularia grisea DNA fingerprint pattern Pathotype Differential cultivar |
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