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| 采用SP法检测商品蛋鸡感染J亚群禽白血病病毒对产蛋性能的影响 | |
| 引用本文： | 徐镔蕊,董卫星,冯小宇,余春明,吕艳丽,孙艳争,李宁,L.F.Lee,Maoxiang Li.采用SP法检测商品蛋鸡感染J亚群禽白血病病毒对产蛋性能的影响[J].中国家禽,2003,25(6):10-12. |
| 作者姓名： | 徐镔蕊 董卫星 冯小宇 余春明 吕艳丽 孙艳争 李宁 L.F.Lee Maoxiang Li |
| 作者单位： | 1. 中国农业大学动物医学院,北京,100094 2. 安徽省农业委员会畜牧局,合肥,230001 3. Avian Disease and Onco1ogy Lab.USDA.East Lansing,MI 48823,USA |
| 摘 要： | 本研究属世界上首次报道J亚群禽白血病病毒感染对蛋鸡产蛋性能的影响。尸体剖检观察到产蛋鸡的生殖系统发育不良，表现为卵巢、输卵管幼稚型，输卵管粗细不均。组织病理学观察，发现卵巢组织中有大量的胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞。用特异性抗J亚群禽白血病病毒(ALV—J)囊膜糖蛋白gp85的单克隆抗体检测不产蛋鸡的卵巢、输卵管等待检的组织切片，均检出病毒阳性抗原。结果表明ALV—J的感染造成产蛋鸡卵巢、输卵管发育不良，是蛋鸡不产蛋的直接原因。 |
| 关 键 词： | SP法检 商品蛋鸡 感染 J亚群禽白血病病毒 产蛋性能 单克隆抗体 免疫组化 |
| 修稿时间： | 2003年1月6日 |
Detecting the Effect on Laying Performance of Egg-type Chickens Infected with Avian Leukosis Virus Subgroup J using SP Test |
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| L.F.Lee,Maoxiang Li.Detecting the Effect on Laying Performance of Egg-type Chickens Infected with Avian Leukosis Virus Subgroup J using SP Test[J].China Poultry,2003,25(6):10-12. | |
| Authors: | LFLee Maoxiang Li |
| Abstract: | This study is the first report about the effection of productivity of egg-chickens infected with ALV-J.By autopsy it was found that maldevelopment was present in the reproduction systems of laying hens,and ovary,oviduct was infantile type.The oviducts develop slightly,but diameter of oviduct was different.By histopathology examining,it was found that tumour cells with conspicuous eosinophilic granules in the ovary.Positive antigens were detected in checked slides with special monoclonal antibody against gp85of ALV-J ,It revealed that maldevelopment of ovary and oviduct infected by ALV-J was direct reason that laying hens did not lay. |
| Keywords: | laying performance avian leukosis virus subgroup J monoclonal antibody immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |