盐胁迫对番茄幼苗转化酶表达及糖代谢的影响

以栽培番茄(Ly cop ersicon escu lentum M ill.)品种中杂9号6叶期幼苗为试验材料,在水培条件下研究了50,100,200,400 m g/L N aC l胁迫处理对番茄幼苗根系和叶片转化酶活性表达,及叶片中葡萄糖、果糖、蔗糖和淀粉代谢的影响。结果表明,转化酶的表达水平与盐胁迫强度和时间有密切关系,根系中转化酶在50 m g/LN aC l胁迫后5和11 d,或100 m g/L N aC l胁迫5和13 d均有极显著高表达;200和400 m g/L N aC l胁迫后11 d有极显著表达;叶片对微盐和高盐伤害的抵御反应强烈,转化酶在50和400 m g/L N aC l胁迫后11 d有显著表达。与叶片中转化酶表达的胁迫强度和胁迫时间相关,叶片中葡萄糖、果糖和蔗糖水平提高;胁迫后至第11天,50,100和200 m g/L N aC l处理叶片中淀粉含量增幅随胁迫强度的增加而增大,而400 m g/L N aC l处理的增幅较小。

Effects of salinity stress on invertase expression and carbohydrate metabolism in tomato seedling

The effects of invertase expression and carbohydrate metabolism in tomato(cv.Zhongza No.9) plant were studied under hydroponic culture with different levels of salinity stress by 50,100,200,400 mg/L of NaCl treatment respectively.The results showed that invertase expression was closely related to both the strength and the duration of salinity stress.Invertases in root were most significantly increased at the 5th and 11th day stress of 50 mg/L NaCl or at the 5th and 13th day stress of 100 mg/L NaCl.They were most significantly increased at the 11th day after stress of 200 and 400 mg/L NaCl.The leaf was strongly resistant to the harm induced by low salinity level and high salinity level.Salinity stress at 50,400 mg/L increased leaf invertase in 11 d.The increase of the leaf glucose,fructose and sucrose was correlated with invertase expression.The starch level in leaf was increased more by 50,100,200 mg/L treatment and less by 400 mg/L treatment in the duration of stress.