枯草芽孢杆菌挥发产物的杀线虫活性评价及成分鉴定

寄牛线虫Ditylenchus destructor是许多作物和蔬菜的重要病原物.本研究进行的密封皿内测定结果表明,由枯草芽孢杆菌BL02和G8产生的挥发物能够强烈地抑制D.destructor(24 h杀虫活件均达100%).经挥发物处理后,大部分线虫在1～12 h内活动逐渐减弱,24 h后完全停止活动.经固相微萃取并经气相色谱-质谱联脂技术进一步鉴定,挥发物包括烷烃、醇、酯、酮、酸、胺、肟、酚及杂环类.本研究为微生物活性挥发物的开发和利用提供了有价值的参考并预示了细菌挥发物在未来植物线虫病害生物防治方而的应用潜力.

Evaluation and Identification of the Potential Nema-ticidal Volatiles Produced by Bacillus subtilis

Plant-parasitic nematode Ditylenchus destructor is one of the most serious pathogens of crops and vegetables. In this study, volatiles produced by two Bacillus subtilis strains BL02 and G8 both displayed 100% nematicidal activity (NA) against D. destructor in sealed dishes. When exposed to these nematicidal volatiles, the nematodes gradually reduced movement within 1-12 h and stopped completely after24 h. The effective volatiles were extracted using solid-phase micro-extraction (SPME) and further identified by gas chro-matography-mass spectrometry (GC-MS) technique. The detected volatile organic compounds (VOCs) included alkyls, alcohols, esters, ketones, acid, amine, oxime, phenols and heterocyclic compounds. This study provided useful information for the exploitation of microbial bioactive volatiles and was the first report about the potential application of bacterial volatiles in biocontrol of plant-parasitic nematode.