| PRL-3及其同源异形体重组杆粒的构建和鉴定 |
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| 引用本文： | 何太平,熊亮,莫丽儿,梁念慈. PRL-3及其同源异形体重组杆粒的构建和鉴定[J]. 湛江医学院学报, 2008, 26(5) |
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| 作者姓名： | 何太平 熊亮 莫丽儿 梁念慈 |
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| 作者单位： | 广东医学院;广东医学院 |
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| 基金项目： | 广东省科技计划 ， 广东省中医药管理局课题 |
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| 摘 要： | 目的构建PRL-3及其同源异形体重组杆状病毒表达载体,为下一步表达PRL-3及其同源异形体蛋白并从中草药活性成分中筛选出PRL-3抑制剂奠定基础。方法应用RT-PCR方法从高转移卵巢癌细胞HO-8910PM扩增PRL-3及其同源异形体编码序列,克隆到供体质粒pFastBac HTB,在DH10Bac菌中进行同源重组,经3种抗生素和蓝白斑筛选,得到Bacmid-HTB-PRL-3和Bacmid-HTB-PRL-3-variant,PCR鉴定其正确性。结果与结论成功构建了PRL-3及其同源异形体重组杆粒。
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| 关 键 词： | PRL-3 杆状病毒 基因重组 |
Construction of PRL-3 gene recombinant baculovirus vector |
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| HE Tai-ping,XIONG Liang,MO Li-er,LIANG Nian-ci. Construction of PRL-3 gene recombinant baculovirus vector[J]. Journal of Guangdong Medical College, 2008, 26(5) |
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| Authors: | HE Tai-ping XIONG Liang MO Li-er LIANG Nian-ci |
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| Abstract: | Objective To construct recombinant Bacmid of PRL-3 and its variant.Methods PRL-3 and its variant cDNA were amplified from total RNA of HO-8910PM by RT-PCR,and inserted into plasmid pFastBacHTB.The recombinant Bacmid of PRL3 and its variant was screened by three antibiotics and Bluo-gal,confirmed by PCR.Results and Conclusion The recombinant Bacmid of PRL-3 and its variant had been successfully constructed.It provides a basis for further study of expression of PRL-3 and its variant proteins and screening small molecule inhibitors from active components of Chinese traditional and herbal drugs. |
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| Keywords: | PRL-3 |
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