| 鹅细小病毒NS2基因的克隆和序列分析 |
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| 引用本文： | 邢明伟,王君伟,贺云霞,布日额. 鹅细小病毒NS2基因的克隆和序列分析[J]. 中国预防兽医学报, 2003, 25(3): 195-197 |
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| 作者姓名： | 邢明伟 王君伟 贺云霞 布日额 |
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| 作者单位： | 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江省“十五”攻关课题 (GB0 1B5 0 2_0 2 ) |
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| 摘 要： | 本研究参考CerBank发表的GPV B株全基因序列，设计并合成一对引物，通过PCR技术扩增出GPV H1株NS2基因，目的片段长约1．4kb，将目的片段克隆到pMD18-T载体后进行序列测定和分析，结果表明：GPV H1株NS2基因全长1356bP，编码451个氨基酸，与国外已发表的GPV B株相比核苷酸序列同源性为98．75％，推导氨基酸序列同源性为98．67％。
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| 关 键 词： | 鹅细小病毒 NS2基因 克隆 序列分析 |
| 文章编号： | 1008-0589(2003)03-0195-03 |
| 修稿时间： | 2002-09-13 |
Cloning and sequencing of NS2 gene of goose parvovirus |
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| XING Ming_Wei,WANG Jun_wei,HE Yun_xia,Bu Ri_e. Cloning and sequencing of NS2 gene of goose parvovirus[J]. Chinese Journal of Preventive Veterinary Medicine, 2003, 25(3): 195-197 |
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| Authors: | XING Ming_Wei WANG Jun_wei HE Yun_xia Bu Ri_e |
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| Abstract: | According to the sequence of GPV B strain published in GenBank, a pair of primers were designed by Oligo4.0.NS2 gene of GVP and amplified by PCR. The gene of interest was cloned into the vector pMD18_T and sequenced. The results showed that NS2 gene consisted of 1356 nucleotides and encoded 451 amino acids. The NS2 gene shared 98.75 % and 98.67 % homology with that of B isolate at nucleotide and amino acid level, respectively. |
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| Keywords: | GPV NS2 gene cloning sequence analysis |
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