| 陆地棉多标记基因系SSR分析 |
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| 引用本文： | 孙志栋,王学德,倪西源.陆地棉多标记基因系SSR分析[J].中国农学通报,2005,21(2):15-15. |
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| 作者姓名： | 孙志栋 王学德 倪西源 |
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| 作者单位： | 1. 浙江大学农学系,杭州,310029;宁波市农科院,浙江,315040
2. 浙江大学农学系,杭州,310029 |
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| 基金项目： | 国家自然科学基金(30170058)、国家转基因植物研究与产业化开发专项(J00-B-002-10)、国家高技术研究发展计划(863计划,2001AA212081)、教育部科技研究基金(重大0114)资助项目. |
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| 摘 要： | 对陆地棉品种T582和T586两个多标记基因系及其杂交后代F1进行了SSR分析。结果表明，从50对SSR引物中筛选出6对引物具有多态性，占总引物数的12%。SSR序列位点多数呈共显性标记，少数为显性标记。SSR序列的扩增与两端设计引物有很大关系。
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| 关 键 词： | 陆地棉 多标记基因系 SSR 分析 |
Analysis of SSR on Tow Multiple-marker Lines in Upland Cotton |
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| Sun Zhidong,Wang Xuede,Ni Xiyuan.Analysis of SSR on Tow Multiple-marker Lines in Upland Cotton[J].Chinese Agricultural Science Bulletin,2005,21(2):15-15. |
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| Authors: | Sun Zhidong Wang Xuede Ni Xiyuan |
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| Institution: | (1Department of Agronomy, Zhejiang University, Hangzhou 310029；2Ningbo Academy of Agricultural Sciences, Ningbo 315040) |
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| Abstract: | Polymorphism analysis of simple sequence repeats (SSR) was carried out for parents, T582 and T586, and their F1 plants. The result of SSR analysis showed that six out of fifty pairs of primers produced DNA polymorphism, which accounts 12 % of the total. The SSR bands were more co-domination loci than domination loci. SSR-motif with DNA polymorphism was related to primers of two ends designed. |
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| Keywords: | Upland cotton Multiple-marker lines SSR Analysis |
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