Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt |
| |
| Authors: | Said Amer Shereif Zidan Yaoyu Feng Haileeyesus Adamu Na Li Lihua Xiao |
| |
| Institution: | 1. Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA, USA;2. Department of Zoology, Faculty of Science, Kafr El sheikh University, Kafe El Sheikh, Egypt;3. Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Sadat Branch, Menofia University, Al Menofia, Egypt;4. School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, People''s Republic of China;5. Addis Ababa University, P.O. Box 42524, Addis Ababa, Ethiopia |
| |
| Abstract: | Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl–Neelsen stain. Cryptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IIdA20G1 (in 5 specimens) and IIaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family IId of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
