Detection of a novel genotype of Cryptosporidium in Antarctic pinnipeds |
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| Authors: | Claudia Rengifo-Herrera Luis Miguel Ortega-Mora Mercedes Gómez-Bautista Francisco Javier García-Peña Daniel García-Párraga Susana Pedraza-Díaz |
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| Affiliation: | 1. SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain;2. Laboratorio Central de Veterinaria de Algete, Ministerio de Medio Ambiente y Medio Rural y Marino, Ctra Madrid-Algete km 8, 28110 Algete, Spain;3. L’Oceanogràfic, Ciudad de las Artes y de las Ciencia, Junta de Murs i Valls s/n, 46013 Valencia, Spain |
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| Abstract: | A study was conducted to investigate the presence of Cryptosporidium and Giardia in Antarctic marine mammals. A total of 270 faecal samples from different species of pinnipeds from different locations in the South Shetland Islands and Antarctic Peninsula were analysed by immunofluorescence microscopy and PCR. Cryptosporidium was detected by PCR in three samples from Southern elephant seals (Mirounga leonina) and 2 Weddell seals (Leptonychotes weddellii). However, no oocysts were observed in any of the samples by immunofluorescence microscopy. Molecular characterisation of the isolates, using the 18S rDNA, the HSP70 and the COWP loci, revealed the presence of a Cryptosporidium sp., previously reported from an Antarctic Southern elephant seal, in the elephant seals and a novel genotype in Weddell seals. Giardia could not be detected in any of the samples analysed. |
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