| 建鲤FABP3基因分离及其多态性与增重的相关分析 |
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| 引用本文： | 俞菊华,李红霞,李建林,唐永凯,夏正龙,董在杰.建鲤FABP3基因分离及其多态性与增重的相关分析[J].水产学报,2012,36(12):1809-1818. |
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| 作者姓名： | 俞菊华 李红霞 李建林 唐永凯 夏正龙 董在杰 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心,农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081 |
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| 基金项目： | 水产分子育种共性技术的建立与应用，国家"八六三"高技术研究发展计划，现代农业产业技术体系建设专项 |
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| 摘 要： | 为获得与建鲤增重相关的分子标记进行建鲤分子育种,实验在建鲤心肌型脂肪酸结合蛋白(FABP)基因上筛选了与增重相关的SNP位点.首先使用PCR扩增到2个建鲤FABP3基因,jlFABP3a和3b基因,两个基因均有4个外显子和3个内含子组成,3a和3b阅读框相似性为93％,编码133个氨基酸,相似性为94％,内含子长度和序列存在着明显差异.jlFABP3的系统进化和传统的分类地位一致.通过序列比对,在3a和3b上分别找到26和25个SNP位点.构建PCR-RFLP法检测了其中3个SNPs在建鲤群体中的分布,并与增重进行相关分析,结果显示,C30G与检测群体雌、雄鱼鱼种阶段和雌鱼成鱼阶段增重显著相关(P＜0.05),CC型个体增重显著快于CG型个体.G267T在5个家系中与雌鱼成鱼增重相关,在7个家系中不同基因型个体增重虽呈相同趋势但差异不显著(P＞0.05).同时考虑G267T和C30G位点,则发现GGCC个体在雌、雄鱼增重均最大,在雌、雄鱼中分别比GGCG个体快17％和12％.GGCC个体在实验样本中占约9％,存在着较大的选育空间.
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| 关 键 词： | 建鲤 心肌型胞内脂肪酸结合蛋白 单核苷酸多态性 SNP-增重相关分析 |
| 收稿时间： | 2/9/2012 12:00:00 AM |
| 修稿时间： | 2012/6/15 0:00:00 |
Sequences analysis of jlFABP3s and the correlation between polymorphisms and body weight gain in Carpio cyprino var. jian |
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| YU Ju-hu,LI Hong-xi,LI Jian-lin,TANG Yong-kai,XIA Zheng-long and DONG Zai-jie.Sequences analysis of jlFABP3s and the correlation between polymorphisms and body weight gain in Carpio cyprino var. jian[J].Journal of Fisheries of China,2012,36(12):1809-1818. |
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| Authors: | YU Ju-hu LI Hong-xi LI Jian-lin TANG Yong-kai XIA Zheng-long and DONG Zai-jie |
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| Institution: | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences |
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