| 甘蓝型油菜蛋白质双向电泳体系的建立 |
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| 引用本文： | 甘露,李殿荣,臧新,付春华,余龙江,栗茂腾.甘蓝型油菜蛋白质双向电泳体系的建立[J].作物学报,2010,36(4):612-619. |
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| 作者姓名： | 甘露 李殿荣 臧新 付春华 余龙江 栗茂腾 |
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| 作者单位： | 1.华中科技大学生命科学与技术学院,湖北武汉430074;2.分子生物物理教育部重点实验室,河北武汉430074;3.陕西省杂交油菜研究中心,陕西大荔715105;4.郑州大学生物工程系,河南郑州450001 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目，新世纪优秀人才支持计划和华中科技大学自主创新基金 |
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| 摘 要： | 选用油菜品种08127，采用TCA-丙酮提取方法，pH 4~7胶条和改进的IEF聚焦程序，得到较好的蛋白质双向电泳结果，建立了一种适合甘蓝型油菜的双向电泳体系；利用其他甘蓝型油菜品种的幼苗、叶片和茎中的蛋白检验了建立的实验体系，都能得到较好的蛋白质双向电泳结果；利用该实验体系比较甘蓝型油菜幼苗不同发育时期蛋白质双向电泳图，找到二者之间的差异点，选取部分点成功进行了质谱分析。
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| 关 键 词： | 甘蓝型油菜 蛋白质 双向电流 |
| 收稿时间： | 2009-09-08 |
Construction of Protein Two-Dimensional Polyacrylamide Gel Electrophoresis System for Brassica napus |
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| GAN Lu,LI Dian-Rong,ZANG Xin,FU Chun-Hua,YU Long-Jiang,LI Mao-Teng.Construction of Protein Two-Dimensional Polyacrylamide Gel Electrophoresis System for Brassica napus[J].Acta Agronomica Sinica,2010,36(4):612-619. |
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| Authors: | GAN Lu LI Dian-Rong ZANG Xin FU Chun-Hua YU Long-Jiang LI Mao-Teng |
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| Institution: | Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 43007, China; Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan 430074, China; Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, China; Bioengineering Department of Zhengzhou University, Zhengzhou 450001, China |
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| Abstract: | Two-dimensional polyacrylamide gel electrophoresis has been widely applied in proteomic researches. The approach can analyze dynamic changes of proteomics of biological samples under different conditions to identify specific proteins and genes. The two-dimensional gel systems for rice,Arabidopsis,and other species have been established. The purpose of this re-search was to establish a suitable system for B. napus to analyze proteomics. Rapeseed variety of 08127 was used in the experi-ment,the proteins were ... |
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| Keywords: | Brassica napus Protein Two-dimensional polyacrylamide gel electrophoresis |
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