In vitro growth of Nidularium minutum mez (Bromeliaceae) in different concentrations of nitrogen,phosphorus, potassium,and calcium

Nidularium minutum Mez is a terrestrial bromeliad with ornamental potential, especially as a species that develops the shade. This, as well as many other bromeliads, is endangered. We studied the growth of nitrogen (N). N. minutum cultured in vitro in different concentrations of nitrogen (N), phosphorus (P), potassium (K), or calcium (Ca) to develop an ideal growth environment for this plant to preserve the species. Results favorable to the growth were treatments containing 60 mM N, 0.60 and 1.25 mM P, 10 mM K, and 1.5 mM Ca. Based on these results two new culture media were prepared (T1 and T2) and were compared with the MS medium with dilutions of macronutrients (50%, 20%, 10%, and 5%). The results showed that changes in the MS medium favored growth of N. minutum.