豆渣悬浮发酵转化为秀珍菇多糖的纯度研究

豆渣可用于液体悬浮培养食用菌菌丝体，并且可以进一步提取食用菌菌丝体多糖，但所得食用菌菌丝体多糖的纯度尚不清楚。为了检测其纯度，以麦角甾醇为指标，间接测定生长在豆渣表面的秀珍菇菌丝体含量，并依据对照秀珍菇菌丝体多糖提取率以及发酵豆渣残渣的多糖提取率，推断秀珍菇多糖的纯度。在此基础上，以秀珍菇多糖纯度为指标，以正交试验L9(34)优化培养基配方。极差分析表明，4个因素对提高多糖提取率的影响顺序为麦麸 > 豆渣 > 葡萄糖 > 马铃薯。最优豆渣固体悬浮发酵配方为：葡萄糖3%、豆渣5%（不过滤）、麦麸（使用滤液）0.5%、磷酸二氢钾0.3%、硫酸镁0.15%、VB1 0.01%、酵母粉0.15%，pH自然。发酵7天后，秀珍菇多糖的纯度可达75.27%，得率为发酵豆渣残渣质量的3.67%。

Research on the Purity of Polysaccharide of Pleurotus geesteranus Extracted from Suspension Fermentation

Although soy dreg, which was the leavings of making tofu, which could be used to cultured edible fungi by suspension fermentation and further to extract the polysaccharide, the purity of polysaccharide prepared by this method was still unknown. In this research, the fungal biomass grown on the surface of soy dreg particles was estimated by the ergosterol contents, and the purity of polysaccharide of Pleurotus geesteranus was estimated based on the yield of polysaccharide from controlled mycelia of Pleurotus...