Bacillus subtilis fmbj与2-脱氧葡萄糖协同作用Rhizopus stolonifer的生物防治效果

 【目的】通过添加佐剂2-脱氧葡萄糖（2-DOG）的方法提高拮抗细菌枯草芽孢杆菌(Bacillus subtilis fmbj)菌株的生防效果。【方法】分别在PDA平板和水蜜桃上测定2-DOG对拮抗菌B.subtilis fmbj和病原菌桃软腐病菌葡枝根霉(Rhizopus stolonifer)生长的影响及B.subtilis fmbj与不同浓度2-DOG协同作用对R.stolonifer的防治效果。【结果】平板试验表明，2-DOG浓度仅为0.3 mg•mL-1时,可完全抑制R.stolonifer的生长；而2-DOG高达20 mg•mL-1，B.subtilis fmbj依然存活,表现出对2-DOG有较高的耐受性。桃上接种试验发现，单独使用浓度为3.9×108 CFU/mL B.subtilis fmbj时，桃果实发病率为30%；4 mg•mL-1 2-DOG单独使用时桃果实发病率为35%；而当4 mg•mL-1 2-DOG和3.9×107 CFU/mL B.subtilis fmbj配合使用时,可完全抑制果实的腐败。【结论】拮抗菌B.subtilis fmbj和2-DOG协同作用比单独使用对桃果实软腐病的防治效果明显，该研究结果为桃果实采后病害控制和保藏提供了有效的方法与途径。

Bio-Control Effect of Combining Bacillus subtilis fmbj with 2-Deoxy-D-Glucose on Rhizopus stolonifer

【Objective】 This study aims to enhance the biocontrol effect of antagonistic Bacillus subtilis fmbj on peach fruit soft rot disease caused by Rhizopus stolonifer.【Method】The synergitic effect of this antagonistic bacterium in combination with different concentrations of 2-Deoxy-D-Glucose (2-DOG) on pathogenic R. stolonifer, and the effect of 2-DOG on growth of both antagonistic bacterium and pathogen were tested in this work. 【Result】 The results in vitro showed that R. stolonifer could be completely inhibited by 0.3 mg•mL-1 of 2-DOG, while B. subtilis fmbj stia grew and gathered abundantly in the wound of peach fruit at 20 mg•mL-1 of 2-DOG and presented a high 2-DOG-resistance. In vivo the results indicated that 4 mg•mL-1 of 2-DOG combined with 3.9×107CFU/mL of B.subtilis fmbj totally protected peach fruit from decay, but when only 3.9×108CFU/mL of B. subtilis fmbj or 2-DOG was used, the soft rot disease of peach fruit would occur and the disease incidence was respectively 30% and 35%.【Conclusion】Combination of B. subtilis fmbj and 2-DOG showed a more effective control on R. stolonifer than applying the B. subtilis fmbj or 2-DOG alone. This research established a significant bio-control method for peach fruit post harvest preservation.