小花棘豆总生物碱对小鼠氧自由基的影响

为了探讨小花棘豆主要有毒成分———总生物碱对动物机体内氧自由基的影响,采用紫外分光光度法分别测定了小白鼠血浆、肝和脑中超氧化物歧化酶(Superoxidedismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)的活性以及脂质氧化代谢终末产物丙二醛(Malonalde-hyde,MDA)的含量。结果表明,攻毒组肝、脑中SOD、GSH-PX活性显著低于对照组(P<0.01),血浆中GSH-PX活性显著低于对照组(P<0.05),SOD活性与对照组则无显著性差异(P>0.05);攻毒组脑、血浆中MDA含量显著高于对照组(P<0.01),但肝脏中的MDA含量却无显著性差异。表明小花棘豆主要有毒成分能显著降低抗氧化酶的活性和提高MDA的含量,并能诱发机体内氧自由基的产生。

Effects of Toxic Components of Oxytopis Glabra DC. on Oxygen Free Radicals in Rats

In order to study the Effects of of Oxytopis glabra DC.on oxygen free radicals,the activities of Superoxide dismutase(SOD),Glutathione peroxidase(GSH-Px) and contents of Malonaldehyde(MDA) were determined by ultra spectrophotometry in plasma,liver and brain of rats.The results demonstrated as follows: The activities of SOD,GSH-Px in lever and brain were markedly lower taking alkaloid group than control group(P<0.01),the activities of GSH-Px in plasma was markedly lower than control group(P<0.05),there were no notable difference in plasma SOD between two groups.The contents of MDA in brain and plasma increased markedly in taking alkaloid group than in control group(P<0.01),but MDA contents in lever were no marked difference between two groups.The results show that the total alkaloid in Oxytopis glabra DC.decreased the activities of SOD and GSH-Px,increased the contents of MDA after taking total alkaloid by oral,because Oxygen Free Radicals were produced in organizers.