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结球甘蓝-大白菜异源三倍体与结球甘蓝回交获得初级三体的研究
引用本文:李艳霞,管志坤,赵建军,申书兴,王彦华.结球甘蓝-大白菜异源三倍体与结球甘蓝回交获得初级三体的研究[J].河北农业大学学报,2012,35(4):37-40.
作者姓名:李艳霞  管志坤  赵建军  申书兴  王彦华
作者单位:河北农业大学园艺学院,河北保定,071000
基金项目:河北省自然科学基金(C2010000677)
摘    要:采用结球甘蓝-大白菜异源三倍体与二倍体结球甘蓝回交的方式,从回交后代BC2中获得了1株2n+1非整倍体植株.经细胞学和SSR鉴定分析,确认该2n+1植株为结球甘蓝8号初级三体.通过对8号初级三体植株自交和回交后代染色体数目的测定,明确了其额外染色体雌、雄配子的传递率分别为34.74%和6.17%.

关 键 词:结球甘蓝  异源三倍体  初级三体  核型分析  SSR

Production of a primary trisomic from progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage
LI Yan-xia , GUAN Zhi-kun , ZHAO Jian-jun , SHEN Shu-xing , WANG Yan-hua.Production of a primary trisomic from progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage[J].Journal of Agricultural University of Hebei,2012,35(4):37-40.
Authors:LI Yan-xia  GUAN Zhi-kun  ZHAO Jian-jun  SHEN Shu-xing  WANG Yan-hua
Institution:(College of Horticulture,Agricultural University of Hebei,Baoding 071000,China)
Abstract:In this study,an aneuploid plant with 2n+1 chromosome was obtained from the progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage.Cytological and SSR analysis showed that the aneuploid plant was a primary trisomic-8 of cabbage.Furthermore,the chromosome number of the selfing and backcrossing progenies of the primary trisomic-8 plant was identified,and the transfer rate of female and male gametes of extra-chromosome was clarified as 34.74% and 6.17%,respectively.
Keywords:cabbage  allotriploid  primary trisomic  karyoptype analysis  SSR
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