月季组培快繁技术研究

通过对５个切花月季品种组培快繁技术的研究，探讨了品种、植物生长调节剂、温度和光照强度等对外植体腋芽萌发、嫩茎增殖和生根的影响。茎段腋芽诱导适宜的条件是：ＭＳ＋ＢＡ３０ｍｇ／Ｌ＋ＮＡＡ０１５ｍｇ／Ｌ，温度２６℃左右。在ＭＳ＋ＢＡ３０ｍｇ／Ｌ＋ＩＡＡ０１５ｍｇ／Ｌ培养基上，嫩茎增殖达４倍以上。３０００ｌｕｘ左右的自然散射光对增殖阶段形成健壮嫩芽有利。小苗移植到沙子与椰糠混合的基质上，成活率达８０％以上。

STUDY ON TECHNIQUE FOR MICROPROPAGATION OF ROSE

Studies on influences of varieties, plant growth regulators, temperature and intensity of illumination on bud germination, mutiplication and rooting were carried out through the micropropagation of 5 varieties of rose. The results show that for growth initiation, the optimum medium is MS+BA 3 0mg/L+NNA 0 15mg/L, and temperature is about 26℃. Shoots cultured on the medium of MS+BA 3 0mg/L+IAA 0 15mg/L could be multiplicated by 4 fold and above. 3000lux of random natural light is useful for robust bud formation. 80% of survival rate could be obtained when plantlets are transplanted to the matrix consisting of sand and coconut husk.