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Bovine Immunodeficiency Virus in Relation to Embryos Fertilized In Vitro |
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| Authors: | Bielanski A Simard C Maxwell P Nadin-Davis S |
| Institution: | (1) Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa-Nepean, Ontario, Canada;(2) Present address: Animal Diseases Research Institute, 3851 Fallowfield Road, PO Box 11300, Station H, Ottawa-Nepean, Ontario, Canada, K2H 8P9;(3) Health of Animals and Food Laboratory, 3400 Boulevard Casavant West, St-Hyacinthe, Quebec, Canada;(4) Centre for Animal and Plant Health, 93 Mount Edward Road, Charlottetown, Prince Edward Island, Canada;(5) Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa-Nepean, Ontario, Canada |
| Abstract: | The association of bovine immunodeficiency virus (BIV) with embryos derived by in vitro fertilization from oocytes of experimentally infected heifers or oocytes/embryos exposed to the virus in vitro was investigated. Using a nested-PCR assay, proviral DNA of BIV was not detected in follicular fluid or in embryos derived from BIV-infected donors. In vitro exposure of oocytes to BIV during maturation or insemination with BIV-infected semen resulted in zona pellucida-intact embryos testing negative for BIV provirus. However, exposure of zona pellucida-free day-7 embryos to the virus resulted in a positive BIV assay for 28% of the batches of embryos, suggesting that the zona pellucida has a role in protecting against BIV infection. The presence of BIV in the IVF system had no apparent effect on the development of bovine embryos to the blastocyst stage. |
| Keywords: | bovine immunodeficiency virus cattle embryos in vitro fertilization maturation oocytes polymerase chain reaction zona pellucida |
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