能量对初情期前母猪卵巢和子宫IGF-1R和EGFR mRNA表达的影响

本实验采用9头“军牧1号”，随机分成三组，分别饲喂高、中、低三个能量组饲料，预饲7日后，进入试验期，试验期维持14日，取下每头母猪卵巢和子宫并用液氮保存。实验结果：在卵巢上，高能组猪IGF-1R、EGFR mRNA的表达量水平显著高于低能组和中能组的表达量（P＜0.05），低能组猪卵巢上这两种生长因子受体表达量要显著低于中能组的( P＜0.05)。在子宫上，高能组猪IGF-1R、EGFR mRNA的表达量要显著高于中、低能组的表达量( P＜0.05)，低能组上这两种生长因子受体表达量要低于中能组的，但差异不显著( P＞0.05)。结果表明：日粮能量水平显著影响初情期前母猪卵巢上两种生长因子受体的表达，高能量对表达有促进作用，有利于两种生长因子作用的发挥，从而利于生殖系统的生长发育；因此，IGF-1R和EGFR可能参与介导能量摄入对初情期前母猪生殖系统发育的影响。

Effects of Dietary Energy Levels on Ovarian and Uterine Expression of IGF-1R and EGFR mRNA in Prepubertal Gilts

Nine prepubertal gilts (JunMu No.1) were randomly allocated to three groups (n=3) and fed with a high level digestive energy (Group H), a low level digestive energy (Group L), and a control group digestive energy (Group M) diet, respectively, for 14d. Free access of water was provided throughout the whole research period. Ovaries and uteruses were collected, after 2 weeks energy treatment, and processed for determination of the absolute quantities of IGF-IR and EGFR mRNA by using real-time PCR. The ovaries and uteruses in the Group H expressed significantly greater amount of IGF-IR and EGFR mRNA than in the Group M and Group L (p＜0.05). But only the ovaries in the Group L expressed significantly less amount of IGF-IR and EGFR mRNA(p＜0.05) than in the Group M. The present study demonstrated that high energy intake markedly enhances the ovarian and uterine expression of IGF-IR and EGFR in prepubertal gilts, whereas, insufficient energy intake markedly inhibits such expressions. The result suggests that IGF-IR and EGFR might be involved in mediating the effects of energy intake on the development of reproductive system in prepubertal gilts.