“青大一号”紫花苜蓿叶蛋白提取研究

[目的]探究"青大一号"紫花苜蓿叶蛋白的提取工艺。[方法]采用酸化加热法配合加盐法提取"青大一号"紫花苜蓿叶蛋白;以单因素试验设计考察料水比、加盐量、pH、预浸时间、絮凝时间、絮凝温度6因素对苜蓿叶蛋白粗蛋白质提取率的影响;同时设计L9(34)正交试验来确定"青大一号"紫花苜蓿的苜蓿叶蛋白最佳提取条件。[结果]"青大一号"紫花苜蓿叶蛋白的最佳提取条件:絮凝温度为70℃,料液比为1∶10,加盐量为1%,絮凝时间为5 min,pH为4。[结论]为紫花苜蓿的工业化生产提供了理论依据。

Study on Extraction Technology of Leaf Protein from Medicago sativa L.cv.Qingda No.1

[ Objective ] The aim was to explore the extraction technology of leaf protein from Medicago sativa L. cv. Qingda No. 1. [ Method ] With Medicago sativa L. cv. Qingda No. 1 as raw material, alfalfa LPC was extracted by acid-heating with salt method. The effects of six single factors like the rate of meal to water, salt concentration, pH, immersion time, flocculation time and flocculation temperature on the extraction rate of LPC crude protein were studied by using single experiment. Based on the results of single factor experiment, L9 （34） orthogonal experi- ment was conducted to study the impact of LPC extraction process and the optimum condition of extracting LPC from Medicago sativa var. Qingda No. 1. [ Result] The optimum conditions for LPC extraction were as followed： the flocculation temperature was 70 ~C ; the flocculation time was 5 min; the solid-liquid ratio was 1：10; the content of salt was 1%, and the pH was 4. [ Conclusion] The research result provides theoretical basis for the industrial production of Medicago sativa.