烟夜蛾气味结合蛋白的同源模建

[目的]预测烟夜蛾(Helicoverpa assulta)的气味结合蛋白。[方法]用同源模建的方法构建烟夜蛾(Helicoverpa assulta)气味结合蛋白的三维结构,并用Procheck、Verify_3D、Errat、ProSa2003等程序进行评价。[结果]程序评价结果表明模建结构是可靠的。模建结构显示烟夜蛾的气味结合蛋白由6个α-螺旋和连接这些螺旋的回折构成,底物结合口袋成锥形;6个保守的半胱氨酸形成3个对结构起稳定作用的二硫键。[结论]预测结构可靠,为烟夜蛾的研究及虫害的防治提供了理论依据。

Homology Modeling of Pheromone Binding Protein of Helicoverpa assulta

[ Objective ] The research aimed to predict three dimension structure of pheromone binding protein of Helicoverpa assulta. [ Meth- od ] The three dimension structure of pheromone binding protein of Helicoverpa asstdta was modeled by homology modeling method. The evalua- tion of the model by Procheck, Verify_3D, Errat and ProSa2003 indicates that the model is reliable. [ Result] The pheromone binding protein of Helicoverpa assulta consists of 6 a-helices and loops between them. 6 conserved cysteines form 3 disulfide bonds can stabilize the structure. [ Conclusion] This study can provide a theoretical basis for the research and pest control of Helicoverpa assulta.