| 鸡新城疫二价油乳剂灭活苗的研制及免疫效果 |
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| 引用本文： | 张晓根,何雷堂.鸡新城疫二价油乳剂灭活苗的研制及免疫效果[J].河南农业大学学报,2001,35(2):182-184. |
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| 作者姓名： | 张晓根 何雷堂 |
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| 作者单位： | 1. 郑州牧业工程高等专科学校,
2. 鹤壁市畜牧兽医工作站, |
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| 摘 要： | 用NDLasota弱毒株、NDV地方分离株（F基因Ⅶ型）-HD1，分别接种鸡胚，收集尿囊液，制备病毒抗原液，福尔马林灭活后按一定比例混合，与白油佐剂乳化成油包水型乳剂疫苗，并对二价苗各项指标进行了测定，证明本疫苗安全、无副作用，接种后3周产生免疫保护，攻毒保护率为100%，接种2周后通过免疫抗体检测，NDHI抗体效价达8log2，疫苗在鸡场应用，获得了满意效果，有效的控制了ND的发生与流行。
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| 关 键 词： | 鸡新城疫 二价疫苗 免疫 抗体 |
| 文章编号： | 1000-2340(2001)02-0182-03 |
Preparation of a divalent oil emulsion killed vaccine against Ne wcastle disease and its immunogenic effects |
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| ZHANG Xiao-gen,HE Lei-tang.Preparation of a divalent oil emulsion killed vaccine against Ne wcastle disease and its immunogenic effects[J].Journal of Henan Agricultural University,2001,35(2):182-184. |
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| Authors: | ZHANG Xiao-gen HE Lei-tang |
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| Abstract: | Chick blastocysts were vaccinated with ND Lasota atte nuated strain and NDV local isolated strain (F gene type Ⅶ)-HD1，respecti vely,followed by collection of allantoic fluid,preparation of viral antigen flui d,proportionate mixing after being killed with formalin,and emulsion into a oil -coated water-type emulsion vaccine with a white oil adjuvant.All technical pa rameters determined of the divalent vaccine showed that it was safe without side effects.Immunological protection happened with 100% challenging protection 3 we eks after vaccination.Examination of immunological antibodies 2 weeks after vacc ination indicated that the antibody titer for NDHI reached 81og2.Application of this vaccine on some breeding chicken farms and laying hen farms has produced sa tisfactory results,effectively keeping Newcastle disease in check. |
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| Keywords: | Newcastle disease divalent vaccine vaccination antibody |
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