| 副猪嗜血杆菌外膜蛋白pilA基因的克隆与表达（摘要） |
| |
| 引用本文： | 叶飞,张培君,田德雨,孙慧玲,王宏俊,龚玉梅,贺云霞.副猪嗜血杆菌外膜蛋白pilA基因的克隆与表达（摘要）[J].农业科学与技术,2011,12(2):195-197. |
| |
| 作者姓名： | 叶飞 张培君 田德雨 孙慧玲 王宏俊 龚玉梅 贺云霞 |
| |
| 作者单位： | 叶飞,YE Fei(北京市农林科学学院,畜牧兽医研究所,北京100097;首都师范大学,生命科学学院,北京100048);张培君,孙慧玲,王宏俊,龚玉梅,贺云霞,ZHANG Pei-jun,SUN Hui-ling,WANG Hong-jun,GONG Yu-mei,HE Yun-xia(北京市农林科学学院,畜牧兽医研究所,北京100097);田德雨,TIAN De-yu(中国农业大学动物医学院,北京,100193) |
| |
| 基金项目： | 国家自然科学基金项目，国家高技术研究发展计划项目，北京市农林科学院青年基金科学项目，北京市农林科学院项目(2010A008).Supported by National Natural Science Foundation of China，National High Technology Research and Development Program of China，Youth Foundation of Beijing Academy of Agriculture and Forestry，Program of Beijing Academy of Agriculture and Forestry |
| |
| 摘 要： | 目的]克隆和表达副猪嗜血杆菌外膜蛋白pilA基因。方法]对已发表的HPS的pilA序列进行序列分析,合成引物,并以HPS血清5型基因组为模板,通过PCR扩增HPS的pilA编码基因,获得目的基因片段;构建重组表达质粒,经IPTG诱导表达至大肠杆菌BI21（DE3）中,进行SDS-PAGE与Westernblot检测。结果]表达的重组蛋白分子质量与预期的43kD一致。结论]为制备亚单位疫苗和诊断试剂奠定了基础。
|
| 关 键 词： | 副猪嗜血杆菌 pilA 克隆 表达 |
Cloning and Expression of pilA Gene of Outer Membrane Protein of Haemophilus parasuis |
| |
| YE Fei,ZHANG Pei-jun,TIAN De-yu,SUN Hui-ling,WANG Hong-jun,GONG Yu-mei,HE Yun-xia.Cloning and Expression of pilA Gene of Outer Membrane Protein of Haemophilus parasuis[J].Agricultural Science ＆ Technology,2011,12(2):195-197. |
| |
| Authors: | YE Fei ZHANG Pei-jun TIAN De-yu SUN Hui-ling WANG Hong-jun GONG Yu-mei HE Yun-xia |
| |
| Institution: | 1.Institute of Veterinary and Husbandry Science,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;2.College of Life Science,Capital Normal University,Beijing 100048;3.College of Veterinary medicine,China Agriculture University,Beijing 100193 |
| |
| Abstract: | Objective] The aim was to clone and express the pilA gene of outer membrane protein of Haemophilus parasuis.Method] The published pilA gene sequence of HPS was analyzed for primer synthesis,and the genome of serotype 5-type of HPS was used as template for PCR amplification of the pilA gene of HPS;the recombinant expression plasmid was constructed and transformed into E.coli BL21(DE3)after induced by IPTG.SDS-PAGE and Western blot analysis were then carried out.Result] The molecular weight of expressed protein was consistent with the expected(43 kD).Conclusion] The results provided a foundation for the preparation of subunit vaccine and diagnostic reagents. |
| |
| Keywords: | pilA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
