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水稻芽期耐冷性的QTL分析
引用本文:杨杰,仲维功,王军,陈志德,曹卿.水稻芽期耐冷性的QTL分析[J].广西农业生物科学,2009(1):46-50.
作者姓名:杨杰  仲维功  王军  陈志德  曹卿
作者单位:江苏省农业科学院粮食作物研究所;国家水稻改良中心南京分中心;
基金项目:国家科技支撑计划重大项目(2006BADO2A03);;江苏省自然科学基金项目(BK2006159);;江苏省农业科技自主创新基金(CX[07]603);;江苏省农业科学院基金项目(6110703;6510806)资助
摘    要:本研究以98个Nipponbare/Kasalath//Nipponbare回交重组自交家系(backcross-inbred lines,BILs)组成的群体为材料,进行水稻芽期耐冷性数量性状基因座的检测和遗传效应分析。25℃正常条件下水稻发芽7d,芽长5~10cm,5℃低温处理10d,之后升温至25℃,缓苗10d,调查活苗率,并以活苗率作为芽期耐冷性的表型值,分析亲本和98个BILs的芽期耐冷性表现。采用Windows QTL Cartographer 1.13a软件的复合区间作图法,共检测到4个苗期耐冷性数量性状基因座(quantative trait locus,QTL),分别位于第3、第7和第12染色体上,命名为qSCT-3-1、qSCT-3~2、qSCT-7和qSCT-12。4个QTL的加性效应分别为11.16、11.14、-8.8和-14.59,可解释表型变异的12.11%,12.66%,6.82%和15.86%。

关 键 词:水稻  芽期  耐冷  QTL定位

Identification of QTL for Rice Plant Cold Tolerance at Early Seedling Stage
Yang Jie Zhong Weigong Wang Jun Chen Zhide Cao Qing Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing Branch of Chinese National Center for Rice Improvement,Nanjing.Identification of QTL for Rice Plant Cold Tolerance at Early Seedling Stage[J].Journal of Guangxi Agricultural and Biological Science,2009(1):46-50.
Authors:Yang Jie Zhong Weigong Wang Jun Chen Zhide Cao Qing Institute of Food Crops  Jiangsu Academy of Agricultural Sciences  Nanjing Branch of Chinese National Center for Rice Improvement  Nanjing
Institution:Yang Jie Zhong Weigong Wang Jun Chen Zhide Cao Qing Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing Branch of Chinese National Center for Rice Improvement,Nanjing,210014
Abstract:Low temperature induced retardation of seedling growth is a common problem in temperate rice growing areas. To gather more information regarding cold-tolerance in rice (Oryza sativa L.), we identified gene loci controlling cold tolerance at early seedling stage. We analyzed the quantitative trait loci (QTL) using 98 backcross-inbred lines (BIL) derived from the backcross between Nipponbare (strongly cold-tolerant rice, as recurrent parent) and Kasalath (cold susceptible rice). A total of 4 QTLs were detecte...
Keywords:Rice (Oryza sativa L    Early seedling stage  Cold-tolerance  QTL mapping
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