喜树幼苗的硝酸还原酶活性及其日变化规律

本文利用体内法测定了喜树幼苗不同器官以及不同叶位叶片的硝酸还原酶活性(NRA),同时观察了喜树幼苗不同叶位叶片的NRA的日变化规律,并考查了NRA与叶面积及比叶重的相关性。结果表明,与根、茎及茎尖中的NRA相比,叶片中的NRA最高。不同叶位叶片的NRA有明显的差异,在所测定的10个不同叶位的叶片中,上数第4~6片叶片的NRA较高。叶片NRA有明显的日变化,呈单峰曲线,峰值出现在中午1230前后。同时,结果表明NRA与叶面积以及比叶重无明显的相关关系。研究结果为进一步研究喜树的氮代谢提供基础资料。图5参18。

Nitrate reductase activity and its diurnal variation rhythm for Camptotheca acuminata seedlings

Nitrate reductase activity(NRA)in different plant organs and leaves in different positions of Camptotheca acuminata seedlings was determined by an In vivo assay, the diurnal variation rhythm of NRA in leaves of different positions was observed, and the correlations between leaf NRA, leaf area and lamina mass per unit area (LMA) were also examined. The results showed that NRA in the leaf was significantly highest, compared with that in other organs such as roots, stems and leaves. In this ex-periment, the 10 leaves were selected from the apex to the base of the seedlings in order. The different NRA occurred obviously in leaves of different positions of C. acuminata seedlings from the apex to the base, and NRA was higher in the 4th-6th leaves. The diurnal change rhythm of leaf NRA showed a one peak curve, and maximum NRA value appeared at about midday (at 12:30 or so). No obvious correlations between NRA and leaf area or lamina mass per unit area were observed. This study offered scientific foundation for the further research on nitrogen metabolism of C. acuminata.