| 一株芽孢杆菌的分离、鉴定及其抗菌效果研究 |
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| 引用本文： | 黄剑飞,李健,刘淇,王群. 一株芽孢杆菌的分离、鉴定及其抗菌效果研究[J]. 安徽农业科学, 2008, 36(6): 2321-2323 |
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| 作者姓名： | 黄剑飞 李健 刘淇 王群 |
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| 作者单位： | 上海水产大学生命科学与技术学院,上海,200090;中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东青岛,266071;中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东青岛,266071 |
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| 基金项目： | 科技部社会公益研究专项(2004DIB4JH165) |
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| 摘 要： | 从健康牙鲆肠道内分离到一株芽孢杆菌(H-1)和鳗弧菌W-1株进行拮抗实验,表明该菌对鳗弧菌具有较强的拮抗作用(抑菌直径33.05 mm)。应用形态学、生理生化及16S rDNA序列分析方法对该菌进行了综合鉴定,结果显示,菌株H-1与枯草芽孢杆菌标准菌株同源性高达98%,故将其确定为Bacillus subtilis。
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| 关 键 词： | 芽孢杆菌 抗菌效果 鉴定 |
| 文章编号： | 0517-6611(2008)06-02321-02 |
| 修稿时间： | 2007-10-24 |
Screening, Identification and Antibiotic Effect of a Strain of Bacillus |
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| HUANG Jian-fei. Screening, Identification and Antibiotic Effect of a Strain of Bacillus[J]. Journal of Anhui Agricultural Sciences, 2008, 36(6): 2321-2323 |
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| Authors: | HUANG Jian-fei |
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| Abstract: | A Bacillus strain(H-1) was isolated from the intestinal tract of healthy Paralichthys olivaceus.Antagonistic activity of H-1 was tested against Vibrio anguillarum(W-1).The results showed that H-1 was found to have strong antagonistic activity against W-1(inhibitory zone diameters=33.05 mm).Through study on its morphological,physiological and biochemical characteristics as well as 16S rDNA sequence homology comparison,the strain H-1 was identified as Bacillus subtilis,which was almost the closest relative to the standard strain with more than 98% similar in sequence under the phylogenetic tree. |
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| Keywords: | Bacillus Antibiotic effect Identification |
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