Identification of tightly linked SSR markers for flower type in carnation (Dianthus caryophyllus L.) |
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Authors: | Masafumi Yagi Toshiya Yamamoto Sachiko Isobe Satoshi Tabata Hideki Hirakawa Hiroyasu Yamaguchi Koji Tanase Takashi Onozaki |
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Institution: | 1. NARO Institute of Floricultural Science (NIFS), 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8519, Japan 2. NARO Institute of Fruit Tree Science (NIFTS), 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan 3. Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba, 292-0818, Japan
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Abstract: | Single or double flower type is one of the most important breeding targets in carnation (Dianthus caryophyllus L.). We mapped the D 85 locus, which controls flower type, to LG 85P_15–2 using a simple sequence repeat (SSR)-based genetic linkage map constructed using 91 F2 progeny derived from a cross between line 85–11 (double flower) and ‘Pretty Favvare’ (single flower). A positional comparison using SSR markers as anchor loci revealed that the map positions of the D 85 locus corresponded to the single locus controlling the single flower type derived from wild D. capitatus ssp. andrzejowskianus. We identified four co-segregating SSR markers on the D 85 locus. Verification of the SSR markers in commercial cultivars revealed that two of the four SSR markers (CES0212 and CES1982) were tightly linked to the D 85 locus, and amplified a 176-bp and 269-bp allele, respectively, which were common and unique to double flower cultivars. The map positions of the D 85 locus and the tightly linked SSR markers will be useful for determining the genetic basis of flower type and for marker-assisted breeding of carnations. |
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