多次传代和致细胞病变猪瘟病毒石门株E2基因序列分析

根据已发表的猪瘟病毒基因组序列，设计合成了2对引物，以脾毒和细胞毒总RNA为模板，利用反转录聚合酶链式反应（RT—PCR）、套式聚合酶链式反应（nPCR）及测序技术，对多次动物传代和致细胞病变的猪瘟病毒的主要外膜糖蛋白E2基因的核苷酸序列进行了测定，用DNA star软件比较分析了E2基因的核苷酸和氨基酸序列的同源性。结果发现，脾毒和标准石门株的核苷酸序列同源性为99．4％，氨基酸序列同源性为99．0％，细胞毒标准石门株的核苷酸序列同源性为98．3％，氨基酸同源性96．7％。由此说明猪瘟病毒经猪多次传代和致细胞病变后均保持遗传的稳定性。

Sequence analysis of E2 gene of poly-passaging and cytopathogenic classical swine fever virus

According to the published CSFV sequece, we designed two pairs of primers; E2 Gene of poly-passaging and Cytopathogenic Clsssical swine fever virus was amplified and sequenced through RT-PCR,n PCR method. The nucletide sequences and the deduced amino sequences of E2 gene were Compared and analyzed by DNA star software. The results showed that the nucleotide homology between spleen virus and Shimen was 99.4 %,the aminoacid homology was 99.0 %, the nucleotide homology between cultured cell virus and Shimen was 98.3 %,the aminoacid homology was 96.7 %, so we educed that after poly-passaging and Cytopathogenic effect, E2 genes of CSFV were genetically stable.