| 马铃薯块茎休眠解除过程的形态学观察与鉴定 |
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| 引用本文： | 文义凯,刘柏林,卢蔚雯,张宁,司怀军,王蒂. 马铃薯块茎休眠解除过程的形态学观察与鉴定[J]. 中国马铃薯, 2013, 0(1): 14-18 |
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| 作者姓名： | 文义凯 刘柏林 卢蔚雯 张宁 司怀军 王蒂 |
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| 作者单位： | 甘肃农业大学生命科学技术学院;甘肃省干旱生境作物学省部共建国家重点实验室培育基地,甘肃省作物遗传改良与种质创新重点实验室甘肃农业大学 |
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| 基金项目： | 国家自然科学基金项目(30871573和31160298) |
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| 摘 要： | 利用石蜡切片分析了室温储存条件下马铃薯栽培品种‘Favorita’块茎休眠解除过程中的形态组织学变化,并对块茎中的淀粉和蛋白质含量的变化作了研究。结果显示,马铃薯块茎在休眠期芽眼分生组织细胞停止分裂,伴随着休眠的解除,芽眼分生组织细胞开始分裂且分裂速度越来越快,芽原基最终形成一个完整的芽,伴随此过程,观察到芽原基周围部分细胞程序性死亡最终发育形成环纹、螺纹导管的现象;马铃薯块茎从休眠解除到芽的萌发过程中淀粉含量则出现下降且淀粉颗粒逐渐由规则卵圆形变为较小的不规则状,在靠近芽原基的分化部位蛋白质含量有明显上升趋势。室温储存60 d的块茎被认为完全解除休眠。
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| 关 键 词： | 马铃薯 块茎 休眠 组织学 细胞学 |
Observation and Identification of Potato Morphological Changes during Tuber Dormancy Release |
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| WEN Yikai,LIU Bailin,LU Weiwen,ZHANG Ning,SI Huaijun,WANG Di. Observation and Identification of Potato Morphological Changes during Tuber Dormancy Release[J]. Chinese Potato, 2013, 0(1): 14-18 |
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| Authors: | WEN Yikai LIU Bailin LU Weiwen ZHANG Ning SI Huaijun WANG Di |
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| Affiliation: | 1.College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China;2.Gansu Provincial Key Laboratory of Aridland Crop Science,Gansu Key Laboratory of Crop Genetics and Germplasm Enhancement,Gansu Agricultural University,Lanzhou,Gansu 730070,China) |
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| Abstract: | The changes in histology,and starch and protein content of potato variety 'Favorita' tuber dormancy breaking process were investigated by paraffin section methods.The results showed that tuber eye meristematic tissue cells stopped dividing in dormancy state and started dividing rapidly along with dormancy breaking.Finally,a bud was formed gradually by bud primordium.The partial programmed cell death around bud primordium eventually formed annular vessel and spiral vessel.The content of starch decreased,and ovoid starch granule changed into small irregular one.The protein content increased dramatically around bud primordium during the period of tuber dormancy breaking to sprouting.It is considered that tuber dormancy break completely 60 days after storage at room temperature. |
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| Keywords: | Solanum tuberosum L. tuber dormancy histology cytology |
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