| 牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素 |
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| 引用本文： | 刘健,刘忠芳,胡小莉,刘绍璞.牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素[J].西南农业大学学报,2010,32(3). |
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| 作者姓名： | 刘健 刘忠芳 胡小莉 刘绍璞 |
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| 作者单位： | 西南大学环境化学研究所; |
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| 基金项目： | 国家自然科学基金资助项目(20875078号) |
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| 摘 要： | 在pH=2.8~4.0的BR缓冲溶液中,硫酸软骨素(CS)由于磺酸基离解而成为带多个负电荷的大阴离子,而低于其等电点(pI=4.7)的牛血清白蛋白(BSA)则以带正电荷的大阳离子存在,两者之间可结合形成复合物.此时将引起共振瑞利散射(RRS)显著增强,并产生新的RRS光谱,其最大散射波长位于304 nm处.与此同时,它的二级散射(SOS)和倍频散射(FDS)也明显增强,且最大SOS和FDS分别位于471 nm和292 nm.散射增强的强度(ΔIRRS、ΔISOS和ΔIFDS)与CS浓度在一定范围内成正比,可用于CS的定量测定.对于CS的检出限(3σ)分别为2.0 ng/mL(RRS),2.9 ng/mL(SOS)和13.2 ng/mL(FDS).研究了适宜的反应条件,考察了共存物质的影响,表明方法有较好的选择性,可用于市售滴眼液中硫酸软骨素含量的测定.
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| 关 键 词： | 共振瑞利散射 共振非线性散射 硫酸软骨素 牛血清白蛋白 |
Determination of Chondroitin Sulfate by Resonance Rayleigh Scattering and Resonance Non-linear Scattering Method with Bovine Serum Albumin |
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| LIU Jian,LIU Zhong-fang,HU Xiao-li,LIU Shao-pu School of Chemistry , Chemical Engineering,Southwest University,Chongqing ,China.Determination of Chondroitin Sulfate by Resonance Rayleigh Scattering and Resonance Non-linear Scattering Method with Bovine Serum Albumin[J].Journal of Southwest Agricultural University,2010,32(3). |
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| Authors: | LIU Jian LIU Zhong-fang HU Xiao-li LIU Shao-pu School of Chemistry Chemical Engineering Southwest University Chongqing China |
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| Institution: | LIU Jian,LIU Zhong-fang,HU Xiao-li,LIU Shao-pu School of Chemistry , Chemical Engineering,Southwest University,Chongqing 400715,China |
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| Abstract: | In a Britton-Robinson(BR) buffer solution of pH 2.8-4.0,chondroitin sulfate(CS),which carries negative charge by the ionization of sulfonic group,reacted with bovine serum albumin,which carries positive charge through electrostatic force and hydrophobic interaction,to form a complex,resulting in a significant enhancement of resonance Rayleigh scattering(RRS) and the appearance of a new RRS spectrum with the maximum wavelength at 304 nm.At the same time,the second-order scattering(SOS) and frequency-doubling... |
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| Keywords: | resonance Rayleigh scattering resonance non-linear scattering chondroitin sulfate bovine serum albumin |
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