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| 高效液相色谱法测定白牦牛乳中VA和VE的含量 | |
| 引用本文： | 常海军,甘伯中,刘长英,梁慧光,余群力,龙瑞军.高效液相色谱法测定白牦牛乳中VA和VE的含量[J].甘肃农业大学学报,2007,42(2):108-111. |
| 作者姓名： | 常海军 甘伯中 刘长英 梁慧光 余群力 龙瑞军 |
| 作者单位： | 1. 甘肃农业大学食品科学与工程学院,甘肃,兰州,730070 2. 甘肃农业大学理学院甘肃,兰州,730070 3. 甘肃农业大学草业学院,甘肃,兰州,730070 |
| 基金项目： | 教育部优秀青年教师科研和奖励基金 |
| 摘 要： | 建立了用高效液相色谱法同时测定白牦牛乳中VA和VE含量的方法.以ODS-C18(5μm,4.6 mm×150mm)为色谱柱,100%的甲醇为流动相,在流速为0.8 mL/min,柱温为25℃条件下,用波长为290 nm的紫外检测器进行检测,分析了白牦牛乳中VA和VE的含量.结果表明:VA的平均检出值为44.458 3μg/(100 mL),标准差(SD)为0.761 4;平均回收率为98.73%,相对标准偏差(RSD)为0.33%;VE的平均检出值为98.527 1μg/(100 mL),标准差(SD)为0.018 4;平均回收率为97.24%,相对标准偏差(RSD)为0.37%. |
| 关 键 词： | 白牦牛 牛乳 维生素A 维生素E 高效液相色谱 |
| 文章编号： | 1003-4315（2007）02-0108-04 |
| 修稿时间： | 2006-09-11 |
Study on content determination of vitamin A and E in white yak's milk by HPLC |
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| CHANG Hai-jun,GAN Bo-zhong,LIU Chang-ying,LIANG Hui-guang,YU Qun-li,LONG Rui-jun.Study on content determination of vitamin A and E in white yak''''s milk by HPLC[J].Journal of Gansu Agricultural University,2007,42(2):108-111. | |
| Authors: | CHANG Hai-jun GAN Bo-zhong LIU Chang-ying LIANG Hui-guang YU Qun-li LONG Rui-jun |
| Institution: | 1. College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;College of Science,Gansu Agricultural University, Lanzhou 730070,China; 3. College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070, China |
| Abstract: | The high performance liquid chromatography method for a simultaneous content determination of vitamin A and vitamin E in white yak's milk has been developed.Separation and contents determination of vitamin A and vitamin E were achieved by using ODS-C18 column(5 μm,4.6 mm×150 mm),methanol(100 %) as mobile phase and at the flow of 0.8 mL/min,column temperature 25 ℃ and 290 nm UV.The results showed that the mean measured amount of vitamin A was 44.4583 μg/100 mL,SD was 0.7614;average recover rate was 98.73 % and RSD was 0.33 %;mean measured amount of vitamin E was 98.5271 μg/100mL,SD was 0.0184;average recover rate was 97.24 % and RSD was 0.37 %. |
| Keywords: | white yak milk vitamin A vitamin E high performance liquid chromatography(HPLC) |
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