Comparative genomic hybridization in detection of DNA changes in canine lymphomas |
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Authors: | Monika Drážovská Katarína Šiviková Ján Dianovský Miroslav Horňák |
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Institution: | 1. Institute of Genetics, University of Veterinary Medicine and Pharmacy in Ko?ice, Ko?ice, Slovak Republic;2. Department of Genetics and Reproduction, CEITEC ‐ Veterinary Research Institute, Brno, Czech Republic |
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Abstract: | In this study, chromosomal imbalances in tumor tissues (lymphomas) and nucleotide changes in tumor suppressor TP53 were studied in a Bernese Mountain dog bitch and a cross breed bitch. Using comparative genomic hybridization, numerous chromosomal rearrangements were detected, which indicated the heterogeneity in tumor growth: in the cross breed bitch, a deletion on the chromosome 9, and duplications on chromosomes 5, 8 and 17 have been found. In the Bernese Mountain Dog bitch, losses on chromosomes 1, 5, 8, 12, 18, 22, 27, 29 and gains on chromosomes 1, 2, 9, 11, 15, 16, 18, 20, 23, 24, 25, 28, 29, 30, 34, 36, 37 and 38 were identified. With the sequencing of the TP53 gene, one silent mutation, transition A/G at position 138 in exon 5 was detected, without changing the amino acid. |
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Keywords: | canine lymphomas chromosomal imbalances comparative genomic hybridization (CGH) PCR technique TP53 gene |
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