Influence of nanochitosan/zeolite composite on growth performance,digestive enzymes and serum biochemical parameters in rainbow trout (Oncorhynchus mykiss) |
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Authors: | Najmeh Sheikhzadeh Mobina Kouchaki Mobina Mehregan Hossein Tayefi‐Nasrabadi Baharak Divband Masoomeh Khataminan Ali Khani Oushani Sadigheh Shabanzadeh |
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Affiliation: | 1. Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;2. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;3. Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Inorganic Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran;5. Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran;6. Department of Fisheries, Babol Branch, Islamic Azad University, Babol, Iran |
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Abstract: | Protective effects of nanochitosan/zeolite composite besides zeolite and chitosan/zeolite composite on rainbow trout growth, digestive enzyme activities and biochemical parameters were studied. Nanochitosan/zeolite hybrid composites with three different ratio of nanochitosan:zeolite (35:100, 3.5:100 and 0.35:100) were synthesized and analysed by X‐ray powder diffraction (XRD), thermal gravim (TG) analysis and scanning electron microscope (SEM) methods. Prepared composites as well as zeolite and control diets were fed to rainbow trout (50 g) for a period of 60 days. The addition of treated diets significantly improved growth performance compared to the control diet. Supplemental zeolite could only enhance the amylase activity in fish intestine, whereas other treatment groups could increase the pepsin activity besides intestinal alkaline phosphatase, trypsin and amylase activities. No differences were observed for intestinal acid phosphatase and lipase activities among the experimental diets. Meanwhile, serum total antioxidant activity and lipid peroxidation product, indicated by malondialdehyde (MDA), significantly increased and decreased, respectively, with some doses of administration, indicating the elevated antioxidant status in treatment groups. Serum‐specific marker enzymes, namely aspartate aminotransferase (AST) and alkaline phosphatase (ALP), were not affected in all groups. Meanwhile serum total protein in most treatment groups was significantly higher than the control group. Results showed that synthesized composites especially nanochitosan/zeolite composite at 5 g/kg had potential to enhance growth performance, digestive enzyme activities and some biochemical parameters in rainbow trout. |
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Keywords: | chitosan composite growth nanochitosan rainbow trout zeolite |
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