| 枯草芽孢杆菌对多环芳烃的降解能力研究 |
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| 引用本文： | 李丽,钟鸣,周启星,李坤,杨铮,姜树坤.枯草芽孢杆菌对多环芳烃的降解能力研究[J].河南农业科学,2007(4):62-64,71. |
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| 作者姓名： | 李丽 钟鸣 周启星 李坤 杨铮 姜树坤 |
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| 作者单位： | 1. 沈阳农业大学,辽宁省农业生物技术重点实验室,辽宁,沈阳,110161
2. 中国科学院,沈阳应用生态研究所陆地生态过程重点实验室,辽宁,沈阳,110161 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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中国科学院重点实验室基金
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辽宁省自然科学基金 |
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| 摘 要： | 从长期受多环芳烃污染的土壤中分离出1株菲降解菌——菌Ⅱ,经生理生化及16S rDNA分析鉴定,该菌株为枯草芽孢杆菌。在单基质菲、芘反应体系中,该菌株具有较强的降解能力。菌Ⅱ不但可以在高浓度的多环芳烃存在下生长良好而且对高浓度多环芳烃有较高的降解能力。多环芳烃与重金属Cu2+的加入对多环芳烃降解菌有很大的影响。在Cu2+浓度小于15 mg/L时,菌Ⅱ能在以多环芳烃为唯一碳源的培养基中生长良好,Cu2+浓度过高将导致菌体死亡。
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| 关 键 词： | 多环芳烃 生物降解 枯草芽孢杆菌 铜离子 |
| 文章编号： | 1004-3268（2007）04-0062-04 |
| 修稿时间： | 2006-12-26 |
Study on Characteristics of Bacillus subtilis strainⅡ for the Degradation of Polycyclic Aromatic Hydrocarbons |
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| LI Li,ZHONG Ming,ZHOU Qi-xing,LI Kun,YANG Zheng,JIANG Shu-kun.Study on Characteristics of Bacillus subtilis strainⅡ for the Degradation of Polycyclic Aromatic Hydrocarbons[J].Journal of Henan Agricultural Sciences,2007(4):62-64,71. |
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| Authors: | LI Li ZHONG Ming ZHOU Qi-xing LI Kun YANG Zheng JIANG Shu-kun |
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| Institution: | 1. Key Laboratory of Agricultural Biotechnology of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, China; 2. Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110161, China |
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| Abstract: | Most of Polycyclic aromatic hydrocarbons(PAHs) are carcinogenesis and environmental pollutant.A phenanthrene-degrading strain,strainⅡisolated from polycyclic aromatic hydrocarbons(PAHs)-contaminated soil was studied and the experiment showed that the strain was able to utilize phenanthrene as the sole carbon source.Based on physical-biochemical characteristics and phylogenetic analysis of 16S rDNA sequence,strainⅡwas identified as Bacillus subtilis.Strain Ⅱwas able to utilize phenanthrene or pyrene as the sole carbon source for growth.After 15 days of cultivation,the concentrations of PAHs could approach 0.PAHs and heavy metal Cu2+ could influence the growth of strainⅡ.Strain Ⅱ could grow well in the medium containing PAHs as sole carbon source when concentrations of Cu2+ was lower than 15mg/L;High Cu2+ concentrations inhibited the growth of strainⅡand even resulted in its death. |
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| Keywords: | Polycyclic aromatic hydrocarbons Biodegradation Bacillus subtilis Cu2 |
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