| 鸡败血霉形体抗原基因的筛选和DNA电泳鉴定 |
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| 引用本文： | 杨百亮,郭建华,赵翠萍,贾东平. 鸡败血霉形体抗原基因的筛选和DNA电泳鉴定[J]. 天津农学院学报, 1999, 0(2) |
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| 作者姓名： | 杨百亮 郭建华 赵翠萍 贾东平 |
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| 作者单位： | 天津农学院动物科学系，山西农业大学动物医学系，天津农学院基础部 |
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| 摘 要： | 应用鸡败血霉形体抗血清筛选鸡败血霉形体Ｓ６株基因文库，得到１７９个阳性克隆，不足重组噬菌体的１％。反复筛选后，获得５个强阳性克隆。抽提ＤＮＡ酶切鉴定，发现２个克隆的外源片段在５ｋｂ左右，另２个在２．５ｋｂ左右，一个为３．６ｋｂ，为特异抗原的制备和基因工程疫苗的研究打下良好基础。
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| 关 键 词： | 鸡败血霉形体 抗原 基因 筛选 鉴定 |
Screening and Determination of Mycoplasma gallisepticum Antigen Gene by DNA Electrophoresis |
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| Yang Bailiang,Guo Jianhua,Zhao Cuiping,Jia Dongping. Screening and Determination of Mycoplasma gallisepticum Antigen Gene by DNA Electrophoresis[J]. Journal of Tianjin Agricultural University, 1999, 0(2) |
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| Authors: | Yang Bailiang Guo Jianhua Zhao Cuiping Jia Dongping |
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| Affiliation: | Yang Bailiang1,Guo Jianhua2,Zhao Cuiping3,Jia Dongping1 |
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| Abstract: | gtll positive clones, less than 1% of the recombinant were selected by screening a M. gallisepticum (MG) genomic DNA library with antisera against MG cells and their membrane proteins. After repeated screening, 5 strong positive clones were selected, 2 clones showing to have about 5 kb inserts, 2 clones showing to have about 2.5 kb inserts and 1 clone showing to have a 3.6 kb insert by electrophoresis. This study may serve as the basis for a vaccine against MG infection and preparation of specific antigen. |
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| Keywords: | M. gallicepticum Antigen Gene Screening Determination |
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