四环素耐药基因在鸡源沙门氏菌中的分布和传播 |
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引用本文: | 王晓泉,王彦红,吴双,焦新安,潘志明,刘秀梵.四环素耐药基因在鸡源沙门氏菌中的分布和传播[J].中国家禽,2007,29(9):10-12,18. |
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作者姓名: | 王晓泉 王彦红 吴双 焦新安 潘志明 刘秀梵 |
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作者单位: | 1. 江苏省人兽共患病学重点实验室,江苏扬州,225009 2. 扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州,225009 3. 江苏省人兽共患病学重点实验室,江苏扬州,225009;扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州,225009 |
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基金项目: | 国家自然科学基金;教育部FANEDD专项基金 |
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摘 要: | 试验分析了84株鸡源沙门氏菌分离株的四环素耐药性,用PCR方法检测四环素耐药基因在分离株中的分布情况。结果显示,四环素耐药率为49%(41/84),鸡白痢和鸡伤寒沙门氏菌仅携带tet(A)基因(23/23),肠炎沙门氏菌和德尔卑沙门氏菌携带tet(A)(8/18)、tet(B)(17/18)或tet(G)(10/18)三种基因,tetC基因在这些沙门氏菌中都没有检测到。该类基因多数位于结合性质粒上,但是不在整合子范围内。
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关 键 词: | 沙门氏菌分离株 四环素耐药基因 鸡 |
修稿时间: | 2007-01-052007-04-23 |
Distribution and Spread of Tetracycline Resistance Genes among Salmonella enterica Isolates from Chicken |
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Institution: | 1.Key Laboratory of Zoonosis of Jiangsu Provinee,Yangzhou,Jiangsu 225009; 2.Key Laboratory of Animal Infeetious Disease,Ministry of Agrieulture, Yangzhou University,Yangzhou, Jiangsu 225009 |
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Abstract: | Eighty-four Salmonella enterica isolates were collected from chickens with clinical signs. 41 of the 84 isolates(49%) were found to be resistant to tetracycline. Of the 41 tetracycline-resistant isolates,the tetracycline-resistant genes were detected by PCR amplification as follows:tet(A)gene was the sole tetracycline-resistant gene found in all the isolates of S. Pullorum and S. Gallinarum;among the strains of S. Enteritidis and S. Derby,8 strains carried a tet(A)gene,17 carried a tet(B),and 10 carried a tet(G)gene. No tet(C) gene was detected in all 41 tetracycline-resistant isolates. The tetracycline-resistant genes located in the conjugation plasmids and were not related to gene cassettes. |
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Keywords: | Salmonella enterica isolates tetracycline-resistant gene chicken |
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